Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒（CSB-E06858Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Col Ⅰ含量。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，本試劑盒通過(guò)高特異性抗體對(duì)實(shí)現(xiàn)靶標(biāo)捕獲與信號(hào)放大，適用于體外研究膠原代謝調(diào)控機(jī)制、評(píng)估纖維化疾病模型進(jìn)展或探索組織工程材料中膠原沉積規(guī)律等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
縮寫(xiě)：

Col Ⅰ
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	90
	Range %	85-96
1:4	Average %	88
	Range %	84-92
1:8	Average %	104
	Range %	100-109

回收率：

The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	99	94-103

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.