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Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

  • 中文名稱(chēng):
    人Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E04799h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒(CSB-E04799h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的Col Ⅲ含量。人Ⅲ型膠原(Col Ⅲ)是人體皮膚中重要的膠原蛋白類(lèi)型,主要存在于嬰兒皮膚和血管內(nèi)膜。其研究背景涉及膠原蛋白的生物學(xué)功能,如支撐皮膚結(jié)構(gòu)、促進(jìn)細(xì)胞遷移和修復(fù)。研究機(jī)制則聚焦于其三螺旋結(jié)構(gòu)、細(xì)胞粘附性及其在皮膚修復(fù)和抗衰老中的作用。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL,適用于體外研究Col Ⅲ在皮膚老化、肝纖維化、心血管疾病或組織工程修復(fù)等領(lǐng)域的表達(dá)調(diào)控,可為探索纖維化疾病分子機(jī)制或評(píng)估抗纖維化藥物效果提供數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 縮寫(xiě):
    Col Ⅲ
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    12.5 ng/mL-800 ng/mL
  • 靈敏度:
    3.12 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-96  
    1:2 Average % 102  
    Range % 97-105  
    1:4 Average % 92  
    Range % 85-98  
    1:8 Average % 97  
    Range % 91-102  
  • 回收率:
    The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 80-94  
    EDTA plasma (n=4) 97 92-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    800 2.850 2.963 2.907 2.778  
    400 2.255 2.203 2.229 2.100  
    200 1.279 1.256 1.268 1.139  
    100 0.659 0.655 0.657 0.528  
    50 0.438 0.475 0.457 0.328  
    25 0.256 0.242 0.249 0.120  
    12.5 0.184 0.172 0.178 0.049  
    0 0.126 0.132 0.129    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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