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  4. Pig Collagen Type Ⅲ,Col Ⅲ ELISA Kit

Pig Collagen Type Ⅲ,Col Ⅲ ELISA Kit

  • 中文名稱：
    豬Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06776p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒（CSB-E06776p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Col Ⅲ含量。Col Ⅲ（膠原蛋白Ⅲ）是細(xì)胞外基質(zhì)的主要成分之一，參與維持肺動(dòng)脈管壁的彈性和完整性。在肺動(dòng)脈高壓（PAH）等疾病中，TGF-β1等因子促進(jìn)PASMCs外膠原沉積，導(dǎo)致肺血管重構(gòu)。低濃度紫杉醇（PTX）可通過抑制TGF-β1/Smad3信號(hào)通路，降低COL Ⅲ表達(dá)，從而逆轉(zhuǎn)纖維化進(jìn)程。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，適用于探究豬模型在纖維化機(jī)制、心血管疾病或皮膚再生等領(lǐng)域的科研需求，為組織工程、病理模型構(gòu)建及分子機(jī)制研究提供可靠數(shù)據(jù)支持，是基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)中膠原代謝相關(guān)課題的理想工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 縮寫：
    Col Ⅲ
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 91-103  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 93  
    Range % 89-95  
  • 回收率：
    The recovery of pig Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 97 92-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    400 1.898 1.878 1.888 1.712  
    200 1.279 1.256 1.268 1.092  
    100 0.834 0.900 0.867 0.691  
    50 0.615 0.635 0.625 0.449  
    25 0.411 0.435 0.423 0.247  
    12.5 0.331 0.355 0.343 0.167  
    6.25 0.298 0.287 0.293 0.117  
    0 0.175 0.177 0.176    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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