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  4. Human Complement fragment 3b,C3b ELISA Kit

Human Complement fragment 3b,C3b ELISA Kit

  • 中文名稱：
    人補(bǔ)體片斷3b(C3b)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09303h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人補(bǔ)體片斷3b(C3b)酶聯(lián)免疫試劑盒（CSB-E09303h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、腦脊液（CSF）、唾液樣本中的C3b含量。C3b是補(bǔ)體系統(tǒng)中的重要分子。其背景在于補(bǔ)體系統(tǒng)是免疫防御的關(guān)鍵部分。研究機(jī)制方面，C3b參與調(diào)理吞噬作用，能與病原體結(jié)合促進(jìn)吞噬細(xì)胞識別吞噬；還參與補(bǔ)體激活的旁路途徑，在免疫調(diào)控、炎癥反應(yīng)等過程中發(fā)揮著重要作用。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，本產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中對補(bǔ)體系統(tǒng)活性的動態(tài)監(jiān)測，例如探究感染性疾病、神經(jīng)炎癥或慢性炎癥模型中C3b的生物學(xué)功能，也可用于評估藥物或生物制劑對補(bǔ)體活化途徑的調(diào)控作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    C3b
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, cerebrospinal fluid (CSF), saliva
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-92
    1:2Average %98
    Range %92-103
    1:4Average %99
    Range %93-103
    1:8Average %94
    Range %89-98
  • 回收率：
    The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-98
    EDTA plasma (n=4)9791-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.115 2.003 2.059 1.932
    1001.748 1.736 1.742 1.615
    501.245 1.266 1.256 1.129
    250.756 0.782 0.769 0.642
    12.50.568 0.572 0.570 0.443
    6.250.333 0.342 0.338 0.211
    3.120.199 0.187 0.193 0.066
    00.128 0.126 0.127 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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