Mouse complement fragment 3b (C3b) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠補(bǔ)體片斷3b(C3b)酶聯(lián)免疫試劑盒（CSB-EQ027757MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3b含量。C3b是補(bǔ)體系統(tǒng)中C3被裂解后產(chǎn)生的重要片段。其背景在于補(bǔ)體系統(tǒng)是機(jī)體免疫防御的關(guān)鍵部分。研究機(jī)制上，C3b參與免疫調(diào)理、補(bǔ)體激活經(jīng)典和旁路途徑等，通過與多種細(xì)胞表面受體結(jié)合，發(fā)揮免疫監(jiān)視、清除病原體等功能。試劑盒檢測范圍為0.235 ng/mL-15 ng/mL，適用于科研場景下評估補(bǔ)體系統(tǒng)激活程度，如炎癥模型動(dòng)物體液樣本分析、免疫相關(guān)疾病機(jī)制研究中C3b動(dòng)態(tài)監(jiān)測，或通過組織勻漿檢測探究局部微環(huán)境補(bǔ)體活性變化，為免疫調(diào)控研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C3b
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.235 ng/mL-15 ng/mL
靈敏度：

0.059 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	103
	Range %	99-105
1:8	Average %	99
	Range %	95-101

回收率：

The recovery of mouse C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	89	86-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.