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  4. Human Cortisol ELISA Kit
  • 中文名稱：
    人皮質(zhì)醇(Cortisol)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05111h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人皮質(zhì)醇(Cortisol)酶聯(lián)免疫試劑盒（CSB-E05111h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、唾液.樣本中的COR含量。人皮質(zhì)醇（Cortisol）是一種由腎上腺分泌的類固醇激素，被稱為“壓力荷爾蒙”，在應(yīng)對(duì)壓力時(shí)發(fā)揮關(guān)鍵作用。它調(diào)節(jié)免疫、代謝和睡眠等生理過程，通過影響血糖、脂肪和蛋白質(zhì)代謝，以及免疫反應(yīng)來幫助身體應(yīng)對(duì)壓力。試劑盒檢測范圍為3.12 ng/ml-200 ng/ml，靈敏度為1.56 ng/ml?？蒲腥藛T可借助該產(chǎn)品開展動(dòng)物模型腎上腺功能評(píng)估、體外藥物干預(yù)實(shí)驗(yàn)的激素分泌分析，或通過唾液樣本進(jìn)行無創(chuàng)式生物節(jié)律研究，適用于實(shí)驗(yàn)室常規(guī)批量檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    COR
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, saliva.
  • 檢測范圍：
    3.12 ng/ml-200 ng/ml.
  • 靈敏度：
    1.56 ng/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
     
  • 線性度：
     
  • 回收率：
     
  • 標(biāo)準(zhǔn)曲線：
     
  • 本試劑盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
      • Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
      • Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
      • Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
      • HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
      • Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
      • Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：生物流體 唾液

樣品信息：人

稀釋比：其他 1：4

產(chǎn)品評(píng)價(jià)： 我用csb-E05111h檢測人類唾液皮質(zhì)醇，od值為1.5285，濃度為23.09，試劑盒整體標(biāo)準(zhǔn)曲線較好，比較穩(wěn)定。

By 王老師

相關(guān)問答

■ 常見問題解答
Q:

I would like to know if corticosterone also affects to OD value.

A:
Thanks for your inquiry.
This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
Q:

I hope you would be able to advice me which kit would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on cortisol. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
One 96T kit can test 43 samples if you run in duplicates.

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