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  4. Human Endostatin,ES ELISA Kit

Human Endostatin,ES ELISA Kit

  • 中文名稱：
    人內(nèi)皮抑素(ES)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07973h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人內(nèi)皮抑素(ES)酶聯(lián)免疫試劑盒（CSB-E07973h）檢測血清、血漿、組織勻漿樣本中的ES含量。人內(nèi)皮抑素（ES）是一種內(nèi)源性抗血管生成蛋白，能特異性抑制血管內(nèi)皮細(xì)胞增殖和遷移，從而抑制腫瘤血管生成。其作用機(jī)制包括調(diào)控內(nèi)皮細(xì)胞表面蛋白表達(dá)、胞內(nèi)信號(hào)通路，以及促進(jìn)內(nèi)皮細(xì)胞凋亡。研究顯示，ES通過抑制VEGF介導(dǎo)的信號(hào)轉(zhuǎn)導(dǎo)和bcl-2/bcl-XL、bad表達(dá)，阻斷血管生成，抑制腫瘤生長和轉(zhuǎn)移。試劑盒檢測范圍為15.6 pg/ml-1000 pg/ml，靈敏度為3.9 pg/ml。適用于腫瘤模型研究、血管生成相關(guān)機(jī)制探索、藥物干預(yù)效果評估等科研領(lǐng)域，例如分析腫瘤組織或血液樣本中 ES 表達(dá)水平與疾病進(jìn)展的關(guān)聯(lián)性，或評估抗血管生成治療對 ES 動(dòng)態(tài)變化的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP
  • 縮寫：
    ES
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/ml -1000 pg/ml
  • 靈敏度：
    3.9 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:200

    Average %

    87

    Range %

    80-94

    1:400

    Average %

    93

    Range %

    88-100

    1:800

    Average %

    92

    Range %

    86-100

    1:1600

    Average %

    90

    Range %

    86-98

  • 回收率：

    The recovery of human ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    89-98

    EDTA plasma (n=4)

    94

    88-100

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    1.976

    2.034

    2.005

    1.827

    500

    1.120

    1.167

    1.143

    0.966

    250

    0.751

    0.728

    0.740

    0.562

    125

    0.494

    0.519

    0.506

    0.329

    62.5

    0.337

    0.321

    0.329

    0.151

    31.2

    0.242

    0.253

    0.247

    0.070

    15.6

    0.221

    0.214

    0.218

    0.040

    0

    0.180

    0.175

    0.178

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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