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  4. Rat Endostatin,ES ELISA Kit

Rat Endostatin,ES ELISA Kit

  • 中文名稱：
    大鼠內(nèi)皮抑素(ES)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07975r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠內(nèi)皮抑素(ES)酶聯(lián)免疫試劑盒（CSB-E07975r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ES含量。大鼠內(nèi)皮抑素（ES）是一種血管生成抑制因子，通過抑制內(nèi)皮細(xì)胞增殖和遷移，發(fā)揮抗血管生成作用，促進(jìn)腫瘤細(xì)胞凋亡。其研究機(jī)制涉及抑制VEGF表達(dá)、抑制新生血管生成，并可能通過血管“正?；弊饔锰岣叻暖熋舾行?。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，在科研領(lǐng)域，本產(chǎn)品可廣泛應(yīng)用于癌癥模型血管抑制效應(yīng)評(píng)估、心血管疾病機(jī)制研究、抗血管生成藥物開發(fā)等基礎(chǔ)研究場景，為探究ES在病理生理過程中的動(dòng)態(tài)變化及其調(diào)控網(wǎng)絡(luò)提供標(biāo)準(zhǔn)化工具，助力血管生物學(xué)、腫瘤學(xué)等領(lǐng)域的基礎(chǔ)科研工作。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP
  • 縮寫：
    ES
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %85-94
    1:2Average %101
    Range %97-104
    1:4Average %90
    Range %87-94
    1:8Average %100
    Range %95-103
  • 回收率：
    The recovery of rat ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-98
    EDTA plasma (n=4)9592-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    4002.531 2.454 2.493 2.341
    2001.889 1.777 1.833 1.681
    1001.208 1.197 1.203 1.051
    500.704 0.687 0.696 0.544
    250.427 0.434 0.431 0.279
    12.50.311 0.327 0.319 0.167
    6.250.239 0.242 0.241 0.089
    00.150 0.154 0.152 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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