Human Glycogen phosphorylase BB,GP-BB ELISA Kit
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中文名稱:人糖原磷酸化酶同工酶BB(GP-BB)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08475h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人糖原磷酸化酶同工酶BB(GP-BB)酶聯(lián)免疫試劑盒(CSB-E08475h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的GP-BB含量。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL,本產(chǎn)品適用于科研領(lǐng)域中心肌損傷相關(guān)機(jī)制研究。該產(chǎn)品可用于心肌缺血?jiǎng)游锬P驮u(píng)估、心臟疾病機(jī)制研究以及藥物干預(yù)效果分析等科研場(chǎng)景,為體外實(shí)驗(yàn)提供定量數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):GP-BB
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.625 ng/mL-40 ng/mL
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靈敏度:0.156 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GP-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 85-98 1:2 Average % 96 Range % 90-102 1:4 Average % 95 Range % 90-101 1:8 Average % 95 Range % 84-102 -
回收率:
The recovery of human GP-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-100 EDTA plasma (n=4) 96 92-105 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 3.047 3.108 3.078 2.901 20 2.475 2.557 2.516 2.339 10 1.839 1.785 1.812 1.635 5 1.128 1.124 1.126 0.949 2.5 0.724 0.719 0.722 0.545 1.25 0.496 0.468 0.482 0.305 0.625 0.330 0.332 0.331 0.154 0 0.177 0.176 0.177 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Evaluation of Glycogen Phosphorylase BB (GPBB) Level in Serum as Marker in Diagnosis of Acute Ischemic Stroke. G AlGawwam,Indian Journal of Forensic Medicine & Toxicology,2021
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