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  4. Human Histone RNA hairpin-binding protein(SLBP) ELISA kit

Human Histone RNA hairpin-binding protein(SLBP) ELISA kit

  • 中文名稱：
    人組蛋白R(shí)NA發(fā)夾結(jié)合蛋白(SLBP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL021372HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人組蛋白R(shí)NA發(fā)夾結(jié)合蛋白(SLBP)酶聯(lián)免疫試劑盒（CSB-EL021372HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SLBP含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)研究領(lǐng)域中細(xì)胞周期調(diào)控機(jī)制探索、組蛋白代謝相關(guān)疾病模型構(gòu)建（如腫瘤發(fā)生機(jī)制研究）、干細(xì)胞分化調(diào)控分析等方向，通過精準(zhǔn)檢測樣本中SLBP表達(dá)水平的變化，為相關(guān)分子機(jī)制研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Hairpin binding protein histone ELISA Kit; HBP ELISA Kit; Histone binding protein ELISA Kit; Histone RNA hairpin binding protein ELISA Kit; Histone RNA hairpin-binding protein ELISA Kit; Histone stem-loop-binding protein ELISA Kit; SLBP ELISA Kit; SLBP_HUMAN ELISA Kit; Stem loop (histone) binding protein ELISA Kit; Stem loop binding protein ELISA Kit
  • 縮寫：
    SLBP
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SLBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of human SLBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9790-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.338 2.398 2.368 2.206
    5001.587 1.599 1.593 1.431
    2500.949 0.968 0.959 0.797
    1250.599 0.589 0.594 0.432
    62.50.425 0.436 0.431 0.269
    31.250.293 0.316 0.305 0.143
    15.60.231 0.222 0.227 0.065
    00.161 0.163 0.162
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.
  • 基因功能參考文獻(xiàn)：
    1. SLBP is a potentially important cellular regulator of HIV-1, thereby establishing a link between histone metabolism, inflammation, and HIV-1 infection PMID: 27454292
    2. showed that inhibiting the SLBP mRNA and protein levels were rescued by epigenetic modifiers suggesting that nickel's effects on SLBP may be mediated via epigenetic mechanisms PMID: 28306745
    3. Cyclin F-mediated degradation of SLBP limits H2A.X accumulation and apoptosis upon genotoxic stress in G2 cell cycle checkpoint. PMID: 27773672
    4. CRL4(WDR23) is required for efficient histone mRNA 3' end processing to produce mature histone mRNAs for translation. CRL4(WDR23) binds and ubiquitylates SLBP in vitro and in vivo, and this modification activates SLBP function in histone mRNA 3' end processing without affecting its protein levels. PMID: 27203182
    5. FEM1 proteins are ancient regulators of Stem-Loop Binding Protein. PMID: 28118078
    6. CRL4-DCAF11 mediates the degradation of SLBP at the end of S phase and this degradation is essential for the viability of cells. PMID: 27254819
    7. arsenic, a carcinogenic metal, decreases cellular levels of SLBP by inducing its proteasomal degradation and inhibiting SLBP transcription via epigenetic mechanisms PMID: 25266719
    8. the S/G2 stable mutant form of SLBP is degraded by proteasome in G1, indicating that indicating that the SLBP degradation in G1 is independent of the previously identified SLBP degradation at S/G2 PMID: 24122909
    9. Although the C-terminal tail of dSLBP does not contact the RNA, phosphorylation of the tail promotes SLBP conformations competent for RNA binding and thereby appears to reduce the entropic penalty for the association. PMID: 25002523
    10. Alternative splicing allows the synthesis of HBP/SLBP isoforms with different properties that may be important for regulating HBP/SLBP functions during replication stress. PMID: 23941746
    11. C-terminal extension of Lsm4 interacts directly with the histone mRNP, contacting both SLBP and 3'hExo. PMID: 24255165
    12. Data suggest that oligomerization and SLBP phosphorylation regulate SLBP-SLIP1-histone-mRNA complex formation/disassociation; sequential and ordered assembly is required. PMID: 23286197
    13. Using yeast two-hybrid screening, the authors identify CT initiation factor-interacting protein (CTIF) as a protein that binds directly to SLBP. SLBP preferentially associates with the CT complex of histone mRNAs consisting of CBP80/CBP20, but not with the eIF4E/eIF4G (ET) complex, as has been proposed. Rapid degradation of histone mRNA on the inhibition of DNA replication requires association of SLBP with CTIF. PMID: 23234701
    14. This paper shows that SLBP is a substrate for the prolyl isomerase Pin1. Pin1, along with PP2A, facilitates dissociation of the SLBP-histone mRNA complex at the end of S-phase, thereby promoting histone mRNA decay and SLBP ubiquitination. PMID: 22907757
    15. This paper describes the biophysical characterization of human SLBP and the SLBP-SLIP1 complex. Human SLBP is an intrinsically disordered protein that is phosphorylated at 23 Ser/Thr sites when expressed in a eukaryotic expression system such as baculovirus. Unphosphorylated human SLBP forms a high affinity heterotetramer with SLIP1 and the SLBP-SLIP1 complex is regulated by SLBP phosphorylation. PMID: 23286197
    16. the crystal structure of a ternary complex of human SLBP RNA binding domain, human 3'hExo, and a 26-nucleotide stem-loop RNA is reported. PMID: 23329046
    17. The nuclear magnetic resonance and kinetic studies presented here provide a framework for understanding how SLBP recognizes histone mRNA and highlight possible structural roles of phosphorylation and proline isomerization in RNA binding proteins PMID: 22439849
    18. SLBP is the only cell cycle-regulated factor required for histone pre-mRNA processing PMID: 12588979
    19. human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle PMID: 15546920
    20. SLBP is imported into the cell nucleus during the cell cycle. PMID: 15829567
    21. SLBP is required for efficient DNA replication probably because a decreased ability to assemble chromatin results in a decrease in the rate of DNA replication PMID: 15916543
    22. Removal of the phosphoryl group from T230 by either dephosphorylation or mutation results in a 7-fold reduction in the affinity of SLBP for the stem-loop RNA PMID: 16492733
    23. replication-dependent histone mRNAs are likely to be the sole target of SLBP PMID: 16931877
    24. SLBP is required for efficient histone 3'-UTR end processing. PMID: 16982637
    25. Here we show that NELF interacts with the cap binding complex (CBC), a factor that plays important roles in mRNA processing steps, and the two factors together participate in the 3' end processing of histone mRNAs, through association with the SLBP. PMID: 17499042
    26. identified five conserved residues in a 15-amino-acid region in the amino-terminal portion of SLBP, each of which is required for translation. PMID: 18025107
    27. Study concludes that the increase in cyclin A/Cdk1 activity at the end of S phase triggers degradation of SLBP at S/G(2). PMID: 18490441
    28. These results suggest a previously undescribed role for SLBP in histone mRNA export. PMID: 19155325

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    SLBP family
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 10904

    OMIM: 602422

    KEGG: hsa:7884

    STRING: 9606.ENSP00000417686

    UniGene: Hs.298345