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  4. Human ICOS ligand(ICOSLG) ELISA kit

Human ICOS ligand(ICOSLG) ELISA kit

  • 中文名稱：
    人ICOS配體(ICOSLG)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL010958HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人ICOS配體(ICOSLG)酶聯(lián)免疫試劑盒（CSB-EL010958HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ICOSLG含量。ICOSLG，也稱為B7-H2或GL50，是一種跨膜糖蛋白，屬于B7配體家族。它在多種抗原呈遞細(xì)胞和非淋巴細(xì)胞上廣泛表達(dá)，作為T細(xì)胞共刺激分子，對(duì)T細(xì)胞的激活、增殖、分化和細(xì)胞因子產(chǎn)生起重要作用。研究機(jī)制涉及ICOSLG與ICOS受體的相互作用，以及其在免疫調(diào)節(jié)和疾病發(fā)生中的作用。試劑盒檢測(cè)范圍為93.75 pg/mL-6000 pg/mL，該產(chǎn)品適用于免疫調(diào)節(jié)機(jī)制研究、腫瘤免疫治療探索以及生物標(biāo)志物分析等科研場(chǎng)景，為探索ICOSLG在免疫系統(tǒng)中的功能及調(diào)控網(wǎng)絡(luò)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD antigen CD275
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    93.75 pg/mL-6000 pg/mL
  • 靈敏度：
    23.4 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %82-94
    1:2Average %96
    Range %89-102
    1:4Average %98
    Range %95-106
    1:5Average %95
    Range %91-100
  • 回收率：
    The recovery of human ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9688-103
    EDTA plasma (n=4)9490-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    60002.748 2.789 2.769 2.592
    30002.375 2.372 2.374 2.197
    15001.834 1.816 1.825 1.648
    7501.061 1.047 1.054 0.877
    3750.632 0.636 0.634 0.457
    187.50.427 0.441 0.434 0.257
    93.750.259 0.269 0.264 0.087
    00.179 0.174 0.177 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, BTN/MOG family
  • 組織特異性：
    Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leuko
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17087

    OMIM: 605717

    KEGG: hsa:102723996

    STRING: 9606.ENSP00000384432

    UniGene: Hs.14155