Human Immunoglobulin A,IgA ELISA Kit

產(chǎn)品詳情

文獻引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒（CSB-E07985h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IgA含量。IgA是一類重要的免疫球蛋白，在黏膜免疫中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)它能阻止病原體黏附，中和毒素等。目前圍繞IgA的研究機制聚焦于其產(chǎn)生、調(diào)節(jié)過程，以及如何利用它開發(fā)針對感染性疾病、自身免疫病等的治療策略。試劑盒檢測范圍為0.24 ng/mL-1000 ng/mL，靈敏度為0.24 ng/mL。適用于科研場景中免疫機制研究、疾病模型分析或生物樣本中IgA水平的動態(tài)監(jiān)測，例如腸道菌群與黏膜免疫關(guān)聯(lián)性研究、慢性炎癥性疾病模型的生物標(biāo)志物檢測等。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

IgA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.24 ng/mL-1000 ng/mL
靈敏度：

0.24 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10000	Average %	89
	Range %	85-94
1:20000	Average %	90
	Range %	86-97
1:40000	Average %	96
	Range %	90-103
1:80000	Average %	89
	Range %	86-93

回收率：

The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-94
EDTA plasma (n=4)	92	88-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進展：

IgA（免疫球蛋白A）是一種免疫球蛋白，分布于人體內(nèi)許多黏膜表面和體液中。IgA是黏膜免疫的主要保護因子，主要通過抗體中和病原體和調(diào)節(jié)腸道微生物群維持腸道健康。此外，IgA還在妊娠期間保護胎兒免受感染，參與自身免疫調(diào)節(jié)，發(fā)揮重要的生物學(xué)作用。