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  4. Rat Immunoglobulin A,IgA ELISA Kit

Rat Immunoglobulin A,IgA ELISA Kit

  • 中文名稱：
    大鼠免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07987r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒（CSB-E07987r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的IgA含量。IgA作為靶點(diǎn)有重要意義。IgA是人體黏膜免疫的主要抗體。研究機(jī)制上，圍繞其與病原體相互作用、在黏膜表面防御機(jī)制等開(kāi)展，旨在通過(guò)干預(yù)IgA相關(guān)通路，研發(fā)治療感染性疾病、自身免疫病等的藥物，為攻克相關(guān)病癥提供新策略。試劑盒檢測(cè)范圍為7.81 ng/mL-2000 ng/mL，本產(chǎn)品適用于免疫學(xué)研究、感染性疾病模型建立、疫苗開(kāi)發(fā)評(píng)估等科研領(lǐng)域，特別適用于長(zhǎng)期監(jiān)測(cè)實(shí)驗(yàn)動(dòng)物體液免疫應(yīng)答動(dòng)態(tài)，或探究免疫調(diào)節(jié)劑、營(yíng)養(yǎng)干預(yù)等外界因素對(duì)大鼠IgA分泌的影響，為免疫相關(guān)機(jī)制研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    IgA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    7.81 ng/mL-2000 ng/mL
  • 靈敏度：
    7.81 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 101  
    Range % 97-104  
    1:2000 Average % 92  
    Range % 88-96  
    1:4000 Average % 90  
    Range % 86-94  
    1:8000 Average % 103  
    Range % 99-107  
  • 回收率：
    The recovery of rat IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 88-98  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.238 0.231 0.235    
    500 0.447 0.424 0.436    
    125 0.692 0.657 0.675    
    31.25 1.224 1.285 1.255    
    7.81 1.798 1.737 1.768    
    0 2.063 2.045 2.054    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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