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  4. Human Interleukin 35(IL-35) ELISA Kit

Human Interleukin 35(IL-35) ELISA Kit

  • 中文名稱：
    人白介素35(IL-35)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13126h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白介素35(IL-35)酶聯(lián)免疫試劑盒（CSB-E13126h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的IL-35含量。IL-35是一種具有重要生物學意義的靶點。它是一種免疫抑制性細胞因子。研究機制方面，IL-35可調(diào)節(jié)免疫細胞功能，抑制炎癥反應。在多種疾病背景下，如自身免疫病、腫瘤等，對其深入研究有望為開發(fā)新的治療策略提供方向。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于科研領(lǐng)域中對IL - 35表達水平的定量分析，例如探索炎癥性疾病、自身免疫疾病模型中的免疫調(diào)控機制，或研究腫瘤微環(huán)境中免疫抑制性細胞因子的動態(tài)變化，為研究IL - 35在免疫穩(wěn)態(tài)維持、慢性炎癥及腫瘤免疫逃逸中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-35
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, cell culture supernates, urine, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 98  
    Range % 94-103  
    1:4 Average % 97  
    Range % 90-106  
    1:8 Average % 94  
    Range % 88-109  
  • 回收率：
    The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.872 2.752 2.812 2.705  
    2000 2.279 2.179 2.229 2.122  
    1000 1.545 1.589 1.567 1.460  
    500 0.952 1.002 0.977 0.870  
    250 0.531 0.575 0.553 0.446  
    125 0.341 0.366 0.354 0.247  
    62.5 0.255 0.275 0.265 0.158  
    0 0.108 0.105 0.107 　  
  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-35 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-35 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

關(guān)于這個人白介素35(IL-35) ELISA Kit（CSB-E13126h）的特異性我有些疑問，請問檢測抗體是專門檢測IL-35還是IL-35的亞基（EBI-3和IL-12p35）？

A:
該試劑盒（CSB-E13126h）是用于檢測IL-35化合物的。試劑盒中的兩種抗體是專門為兩個亞基設(shè)計的。

靶點詳情

  • 最新研究進展：
    IL-35是一種由IL-12家族成員p35和EBI3亞單位組成的細胞因子，它具有免疫抑制和抗炎作用。近年來的研究表明，IL-35在炎癥性和自身免疫性疾病中具有潛在的治療作用。例如，在小鼠實驗中，IL-35能夠抑制自身免疫性疾病的發(fā)生，如自身免疫性腦脊髓炎、炎癥性腸病等。此外，IL-35也被發(fā)現(xiàn)在某些腫瘤中表達水平較高，研究人員嘗試利用其在腫瘤免疫治療中的潛在作用。