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  4. Mouse Interleukin 35(IL-35) ELISA Kit

Mouse Interleukin 35(IL-35) ELISA Kit

  • 中文名稱：
    小鼠白介素35(IL-35)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13145m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠白介素35(IL-35)酶聯(lián)免疫試劑盒（CSB-E13145m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL-35含量。IL-35是IL-12家族的新成員，由EBI3和p35亞基組成。它在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用，與IL12B形成IL-12細(xì)胞因子或與EBI3/IL27B形成IL-35細(xì)胞因子。IL-35在多種炎癥和腫瘤中表達(dá)異常，其作用機(jī)制涉及調(diào)節(jié)T細(xì)胞和自然殺傷細(xì)胞反應(yīng)，以及誘導(dǎo)干擾素γ的產(chǎn)生。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于體外培養(yǎng)細(xì)胞因子分泌分析、實(shí)驗(yàn)動(dòng)物模型血清/組織IL-35動(dòng)態(tài)監(jiān)測(cè)等場(chǎng)景；為研究IL-35在免疫調(diào)控機(jī)制、疾病模型中的功能解析及藥物干預(yù)效果評(píng)價(jià)提供了高效工具，廣泛應(yīng)用于免疫學(xué)、腫瘤學(xué)及炎癥相關(guān)基礎(chǔ)研究領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    IL-35
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %87-97
    1:2Average %95
    Range %89-99
    1:4Average %94
    Range %90-100
    1:8Average %93
    Range %87-97
  • 回收率：
    The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9487-98
    EDTA plasma (n=4)9691-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.461 2.391 2.426 2.235
    5002.073 1.965 2.019 1.828
    2501.669 1.577 1.623 1.432
    1251.272 1.183 1.228 1.037
    62.50.814 0.804 0.809 0.618
    31.20.567 0.525 0.546 0.355
    15.60.335 0.314 0.325 0.134
    00.194 0.188 0.191 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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