Human Interleukin 5,IL-5 ELISA KIT
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中文名稱:人白介素5(IL-5)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04636h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人白介素5(IL-5)酶聯(lián)免疫試劑盒(CSB-E04636h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的IL5含量。IL5 是重要的治療靶點(diǎn)。它主要由 Th2 細(xì)胞等產(chǎn)生,可促進(jìn)嗜酸性粒細(xì)胞的生長、分化、存活及活化。在哮喘等疾病中,嗜酸性粒細(xì)胞過度活化參與炎癥反應(yīng)。針對(duì) IL5 開展研究,旨在阻斷其信號(hào)通路,抑制嗜酸性粒細(xì)胞相關(guān)炎癥,為相關(guān)疾病治療提供新方向。試劑盒檢測(cè)范圍為3.9 pg/ml-250 pg/ml,靈敏度為0.97 pg/ml。適用于科研實(shí)驗(yàn)中IL - 5水平的動(dòng)態(tài)監(jiān)測(cè)。在基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域,可用于評(píng)估疾病模型動(dòng)物血清中細(xì)胞因子表達(dá)譜、分析炎癥微環(huán)境中組織勻漿的免疫調(diào)節(jié)機(jī)制,或體外藥物干預(yù)實(shí)驗(yàn)的療效驗(yàn)證,為免疫調(diào)節(jié)機(jī)制研究及潛在治療靶點(diǎn)探索提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:B-cell differentiation factor I ELISA Kit; Colony stimulating factor ELISA Kit; EDF ELISA Kit; Eosinophil differentiation factor ELISA Kit; IL-5 ELISA Kit; IL5 ELISA Kit; IL5_HUMAN ELISA Kit; Interleukin 5 (colony stimulating factor; eosinophil) ELISA Kit; Interleukin 5 ELISA Kit; Interleukin-5 ELISA Kit; T Cell Replacing Factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:3.9 pg/ml-250 pg/ml
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靈敏度:0.97 pg/ml
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長:450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
88
Range %
82-96
1:2
Average %
92
Range %
90-105
1:4
Average %
95
Range %
92-103
1:8
Average %
90
Range %
86-98
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回收率:
The recovery of human IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
85
80-92
EDTA plasma (n=4)
87
82-94
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
250
2.973
2.834
2.904
2.754
125
2.276
2.167
2.222
2.072
62.5
1.248
1.228
1.238
1.089
31.25
0.635
0.629
0.632
0.483
15.6
0.363
0.371
0.367
0.218
7.8
0.269
0.253
0.261
0.112
3.9
0.217
0.214
0.216
0.066
0
0.144
0.155
0.149
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Determine Role of IL-5 and Eosinophils in Chronic Bronchial Asthma MH Jabbar,/,2023
- The TLR4/NF-κB signaling pathway-mediated type 2 skewing of T helper cell in cough variant asthma was counteracted by ethanol extract of Anacyclus pyrethrum root J Zheng,Immunobiology,2023
- Circulating levels of IL-33 are elevated by obesity and positively correlated with metabolic disorders in Chinese adults H Tang,Journal of Translational Medicine,2021
- In vivo targeting of miR‐223 in experimental eosinophilic oesophagitis AM Collison,Clinical & Translational Immunology,2020
- Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions Ali Abdulqader, et al,Asian Pacific Journal of Tropical Biomedicine,2018
- Impact of intestinal Entamoeba histolytica on sera of Interleukin-5, histamine, Secretory-IgA and amylase Khalil Ismail A. Mohamed.et al,Curr Res Microbiol Biotechnol,2017
- Elderly-onset hereditary pulmonary alveolar proteinosis and its cytokine profile Masayuki Ito.et al,BMC Pulmonary Medicine,2017
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靶點(diǎn)詳情
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功能:Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
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基因功能參考文獻(xiàn):
- IL-5 is expressed intrathecally in tick-borne encephalitis, but its pathogenetic role remains unclear. PMID: 29602685
- eosinophilia in FE is secondary to dysregulation of IL-5 production in PBMC (and their component subsets). PMID: 28226398
- Data indicate that interleukin-5 (IL-5) was that only serum cytokines show statistical significance between severe chronic obstructive pulmonary disease (COPD) and controls. PMID: 28398462
- Serum IL-5 and IL-13 are reliable biomarkers for the blood eosinophilia asthma phenotype. PMID: 27060452
- Longitudinal co-variations between inflammatory cytokines, lung function and patient reported outcomes in patients with asthma PMID: 28915273
- Obesity alters the lung neutrophil infiltration to enhance breast cancer metastasis through IL5 and GM-CSF. PMID: 28737771
- simvastatin was demonstrated to inhibit IL-5-induced CCR3 expression and chemotaxis of eosinophils mediated via the mevalonate pathway. PMID: 27275740
- Interleukin-5/-targeted treatments offer promises to patients with eosinophilic respiratory disorders; review PMID: 26859368
- Data indicate that interleukin 5 (IL-5)-associated single nucleotide polymorphisms (SNPs) were not associated with carotid intima-media thickness. PMID: 26821299
- Post-liver transplant patients with food allergy showed a unique cytokine profile in response to various stimuli, with extremely elevated IL-5, low IL-10 secretion. PMID: 26282695
- Production of IL-5 in response to both extract and the Bet v1-derived peptide mix strongly suggested that T cells were a major source of IL-5. PMID: 25817862
- It has been shown in this study that the level of IL-5, one of the markers of eosinophilic inflammation, is higher in EBC of children with atopic asthma than in those with nonatopic asthma. PMID: 25937050
- IL-5 may be part of protective mechanisms operating in early atherosclerosis, at least in women PMID: 25587992
- Results showed that PAX2 expression is significantly overexpressed in esophageal squamous cell carcinoma tissues and IL-5 is identified as PAX2's effector for metastasis. PMID: 25613757
- Subsequent IL-5-stimulated signaling. PMID: 25121926
- increased levels in recurrent versus nonrecurrent rhinosinusitis with nasal polyps at the moment of the first surgery PMID: 24980230
- HDM-specific IL-5 responses at age 3 years or later are the best measure of T cell function for predicting asthma at age 8 years. PMID: 24875149
- Fibrocytes studied were generated in vitro from PBMCs by culturing in the presence of platelet derived growth factors and stimulated by IL-33. Fibrocytes constitutively expressed IL-13 and IL-5, which was augmented by stimulation with IL-33. PMID: 24822215
- Mechanisms of human eosinophil migration induced by the combination of IL-5 and the endocannabinoid 2-arachidonoyl-glycerol. PMID: 24530098
- Patients with the 'IL-5, IL-17A, IL-25-high' airway inflammatory pattern are often uncontrolled asthmatics, despite daily treatment. PMID: 23957336
- These unexpected results provide a theoretical basis for the therapeutic targeting of IL-5 in bladder cancer PMID: 23770289
- In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
- When patients were classified according to sputum IgE levels, it appeared that IL-5, IL-6, IL-17 and TNF-alpha sputum supernatant levels were raised in the "IgE high" asthmatics when compared to "IgE low" asthmatics PMID: 23555579
- alpha(M)beta(2) integrin-mediated adhesion and motility of IL-5-stimulated eosinophils on periostin. PMID: 23306834
- Type I interferon (IFN)-dependent inhibition of T cell-derived IL-5 is mediated by IFN-alpha acting directly on activated T cells. PMID: 23382558
- Strong IL-5 production was detected in response to peptides from several of the previously undescribed proteins of grass pollen. PMID: 23401558
- Topical steroid treatment may suppress IL-5 gene expression, and steroids may inhibit eosinophil functions in nasal polyps. PMID: 15835818
- Elevated expression of inflammatory cytokines (IL-5, IL-20, and IL-28A) is associated with bladder cancer development. PMID: 22962576
- binding of IL-5 to IL-5Ralpha plays a critical role in MMP-9 expression, which may be involved in the migration of bladder cancer. PMID: 22710862
- The study reports the crystal structure of dimeric IL-5 in complex with the IL5RA extracellular domains. PMID: 22528658
- Elevated levels of IL-5, which uses the neural plasticity-related RAS GTPase-ERK pathway to mediate its actions in the central nervous system, could be one of the factors underlying the depression-related changes in CNS plasticity. PMID: 22230487
- elevated levels in induced sputum from patients with asthma and allergic rhinitis PMID: 22186238
- REVIEW: Interleukin-5 and IL-5 receptor in health and disease PMID: 21986312
- The structure demonstrates that for steric reasons, homodimeric IL-5 can bind only one receptor molecule, even though two equivalent receptor-binding sites exist. PMID: 22153509
- There is no correlation between the eosinophilic infiltration and the expression of IL-5 in lung cancer tissues. PMID: 21609545
- Transplantation of human mesenchymal stem cells suppresses middle cerebral artery occlusion (MCAO) focal ischemia-induced inflammation, possibly through expression of fractalkine and interleukin (IL)-5. PMID: 21168500
- results demonstrate that IL-5(+) and IL-5(-) Th2 cells, respectively, represent more and less highly differentiated Th2 cell subpopulations PMID: 21849680
- IL-5 may play a role in the early phase of acute pneumonia caused by the 2009 H1N1 virus in Japanese children. PMID: 21323726
- Activation of GATA-3 might be one of the mechanisms for induction of IL-5 expression in chronic rhinosinusitis. PMID: 17628972
- AP-1 may participate in the signal transduction of PKC-triggered expression of IL-5 in allergic rhinitis T lymphocytes. PMID: 17438848
- PPD-induced preferential secretion by PBMCs of Warao indigenous patients in Venezuela PMID: 20650294
- Functional polymorphisms in the genes encoding the Th2 cytokines Il-5, IL-6, and IL-13, are associated differently with the development and prognosis of autoimmune thyroid disease from each other. PMID: 21235536
- IL-5 and IL-8 are the key cytokines in the formation of nasal polyps. PMID: 16929824
- Suggest that IL-5 responses in cord blood samples were strongly related to the season of birth. PMID: 20825427
- promotes increased immunoglobulin M at the site of disease in leprosy PMID: 20561085
- Our study identifies CXCL10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries PMID: 20943047
- Sputum IL-5 was associated with a sputum eosinophilia. PMID: 19478474
- data indicate that ability to produce the type 2 cytokines IL-13 and IL-5 defines CD161(+) NK cells at intermediate stages of differentiation, and is lost upon terminal functional differentiation, concomitant with acquired ability to produce IFN-gamma PMID: 11830476
- CD4(+) T cells are the major source of IL-5 among CD3(+) lymphocytes in atopic asthmatic subjects, whereas in nonatopic asthmatic subjects CD8 (+) T cells as well as CD4(+) T cells contribute to the increased production of IL-5 PMID: 11842300
- By itself, IL5 increases nerve growth factor level in eosinophils, but in combination with immune complexes, significantly reduces eosinophil NGF levels. PMID: 11877300
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亞細(xì)胞定位:Secreted.
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蛋白家族:IL-5 family
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