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  4. Human Myeloid Progenitor Inhibitory Factor 1,MPIF-1 ELISA Kit

Human Myeloid Progenitor Inhibitory Factor 1,MPIF-1 ELISA Kit

  • 中文名稱：
    人骨髓抑制因子1(MPIF-1/CCL23)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07309h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人骨髓抑制因子1(MPIF-1/CCL23)酶聯(lián)免疫試劑盒（CSB-E07309h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL23含量。CCL23，又稱巨噬細胞炎癥蛋白3（MIP-3）和骨髓祖細胞抑制因子1（MPIF-1），是一種CC趨化因子家族的小細胞因子，主要來源于肺、肝和胰腺等組織中的巨噬細胞及樹突狀細胞。CCL23通過與趨化因子受體CCR1結(jié)合，激活并趨化單核細胞、巨噬細胞及T淋巴細胞，在免疫應(yīng)答和炎癥反應(yīng)中發(fā)揮重要作用。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，靈敏度為9.7 pg/mL。適用于科研領(lǐng)域中對MPIF-1/CCL23表達水平的動態(tài)監(jiān)測，例如探究其在自身免疫性疾病、慢性炎癥或腫瘤微環(huán)境中的調(diào)控機制，為免疫相關(guān)疾病的基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C C motif chemokine ligand 23 ELISA Kit; C-C motif chemokine 23 ELISA Kit; C6 beta chemokine ELISA Kit; CCL 23 ELISA Kit; CCL23 ELISA Kit; CCL23(30-99) ELISA Kit; CCL23_HUMAN ELISA Kit; Chemokine (C C motif) ligand 23 ELISA Kit; Chemokine CC motif ligand 23 ELISA Kit; ck beta 8 ELISA Kit; CK-beta-8 ELISA Kit; CKB 8 ELISA Kit; CKB-8 ELISA Kit; Ckb-8-1 ELISA Kit; CKB8 ELISA Kit; Hmrp-2a ELISA Kit; Macrophage inflammatory protein 3 ELISA Kit; MIP 3 ELISA Kit; MIP-3 ELISA Kit; MIP3 ELISA Kit; MPIF 1 ELISA Kit; MPIF-1 ELISA Kit; MPIF1 ELISA Kit; Myeloid progenitor inhibitory factor 1 ELISA Kit; OTTHUMP00000163948 ELISA Kit; SCYA 23 ELISA Kit; SCYA23 ELISA Kit; Small inducible cytokine A 23 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys); member 23 ELISA Kit; Small inducible cytokine subfamily A; member 23 ELISA Kit; Small-inducible cytokine A23 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    39 pg/mL-2500 pg/mL
  • 靈敏度：
    9.7 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    309.863

    319.761

    329.703

    315.796

    325.721

    335.691

    SD

    0.035

    0.043

    0.048

    0.038

    0.045

    0.064

    CV(%)

    4.353

    5.218

    5.687

    4.657

    5.383

    7.477

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MPIF-1/CCL23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    80-96

    1:2

    Average %

    92

    Range %

    90-97

    1:4

    Average %

    100

    Range %

    93-109

    1:8

    Average %

    93

    Range %

    85-96

  • 回收率：

    The recovery of human MPIF-1/CCL23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    86

    80-94

    EDTA plasma (n=4)

    95

    92-107

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2500

    2.820

    2.732

    2.776

    2.672

    1250

    2.057

    2.036

    2.047

    1.943

    625

    1.306

    1.291

    1.299

    1.195

    312.5

    0.851

    0.796

    0.824

    0.720

    156

    0.551

    0.531

    0.541

    0.437

    78

    0.372

    0.358

    0.365

    0.261

    39

    0.258

    0.249

    0.254

    0.150

    0

    0.108

    0.099

    0.104

    　

    LOD

    9.7 pg/ml

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
        CCL23是一種趨化因子，已被證明在多種疾病中發(fā)揮重要作用，如哮喘、肺癌和自身免疫性疾病等。研究表明，CCL23在哮喘和COPD患者中的含量顯著增加，并且與疾病的嚴重程度呈正相關(guān)。此外，最近的研究表明，CCL23可以促進腫瘤細胞增殖和轉(zhuǎn)移，因此可能是治療腫瘤的潛在靶點。
     
  • 功能：
    Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
  • 基因功能參考文獻：
    1. CCL23 is posttranslationally modified by trypsin-like serine proteases and CCL23(47-117) might be a major active form of CCL23 in nasal polyps with chronic rhinosinusitis. PMID: 26560043
    2. The coexistence of diabetes and oxidative stress independently affected CCL23 levels, while the presence of Cardiovascular disease and inflammation had no impact on its concentrations. PMID: 24995525
    3. ADAMTS 8, CCL23, and TNFSF15 are implicated in anti-angiogenic activities PMID: 24384427
    4. Data show CCL23 stimulates chemotaxis of human THP-1 monocytes and enhances release of MMP-2, and suggest it plays a role in atherogenesis. PMID: 21656154
    5. Overproduction of CCL23 in nasal polyps might contribute to the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps PMID: 21497884
    6. Patients with systemic sclerosis who had elevated CCL23 levels had shorter disease duration, and a higher frequency of pulmonary arterial hypertension. Serum CCL23 level was increased in early phase of disease and could be a marker for disease activity. PMID: 20824279
    7. Fresh eosinophils contained trace amounts of CCL23 protein. CCL23 was significantly released into the supernatant when the eosinophils were stimulated with GM-CSF or IL-5 but not with IFN-gamma or immobilized sIgA. PMID: 21646793
    8. independently associated with coronary atherosclerosis PMID: 20187767
    9. CKbeta8 transduces the chemotaxis signal through the G(i)/G(o) protein, phospholipase C, protein kinase C delta and NF-kappaB. PMID: 19951712
    10. CCL23 promoted chemotactic migration and differentiation of endothelial cells, and neovascularization in the chick chorioallantoic membrane PMID: 15927850
    11. May play a direct role in angiogenesis via upregulation of matrix metalloproteinase (MMP)-2 expression. PMID: 16378600
    12. Collectively, these data suggest a link between the inducible phenotype of CCL23 expression in monocytes by the prototype Th2 molecule pair IL-4/STAT6 and the increased number of CCL23-expressing cells in skin of atopic dermatitis patients. PMID: 17371990
    13. A peptide ligand termed SHAAGtide is cleaved from CCL23; is itself an attractant of monocytes and neutrophils in vitro; recruits leukocytes in vivo; is more potent than other natural agents with activity for FPRL1; receives authors' designation "CCR12." PMID: 17513790
    14. This protein may participate in the malignant progression of certain human cancer cells that overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. PMID: 18258606
    15. CCL23, M-CSF, TNFRSF9, TNF-alpha, and CXCL13 are predictive of rheumatoid arthritis disease activity and may be useful in the definition of disease subphenotypes and in the measurement of response to therapy in clinical studies. PMID: 18668547
    16. these findings suggest that CCL23 results in up-regulation of KDR/flk-1 receptor gene transcription and protein expression and that KDR/Flk-1 up-regulation induced by CCL23 may contribute to potentiation of VEGF action in angiogenesis. PMID: 19265684

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CC
  • 數(shù)據(jù)庫鏈接：

    HGNC: 10622

    OMIM: 602494

    KEGG: hsa:6368

    UniGene: Hs.169191