Human PRKC apoptosis WT1 regulator protein(PAWR) ELISA kit
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中文名稱:人PRKC凋亡WT1調(diào)節(jié)蛋白(PAWR)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL017486HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人PRKC凋亡WT1調(diào)節(jié)蛋白(PAWR)酶聯(lián)免疫試劑盒(CSB-EL017486HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PAWR含量。PAWR 即 PRKC, Apoptosis, WT1, Regulator,是一種腫瘤抑制基因。它能通過(guò)多種信號(hào)通路調(diào)節(jié)細(xì)胞凋亡、增殖與分化。研究發(fā)現(xiàn)其表達(dá)異常與腫瘤發(fā)生發(fā)展密切相關(guān),深入研究其作用機(jī)制,有望為癌癥等疾病的治療提供新靶點(diǎn)和策略。試劑盒檢測(cè)范圍為25 pg/mL-1600 pg/mL,適用于腫瘤生物學(xué)研究、細(xì)胞凋亡機(jī)制探索、藥物作用靶點(diǎn)篩選等科研領(lǐng)域,尤其適用于PAWR相關(guān)信號(hào)通路的功能研究及其在疾病模型中的表達(dá)水平動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名:2310001G03Rik ELISA Kit; PAR 4 ELISA Kit; PAR-4 ELISA Kit; Pawr ELISA Kit; PAWR_HUMAN ELISA Kit; PRKC Apoptosis WT1 Regulator ELISA Kit; PRKC apoptosis WT1 regulator protein ELISA Kit; Prostate apoptosis response 4 protein ELISA Kit; Prostate apoptosis response protein 4 ELISA Kit; prostate apoptosis response protein PAR-4 ELISA Kit; Transcriptional repressor Par-4-like protein PAWR ELISA Kit; Transcriptional repressor PAR4 ELISA Kit; WT1 Interacting Protein ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測(cè)范圍:25 pg/mL-1600 pg/mL
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靈敏度:6.25 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Epigenetics and Nuclear Signaling
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PAWR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 80-91 1:2 Average % 90 Range % 84-98 1:4 Average % 92 Range % 86-100 1:8 Average % 95 Range % 88-101 -
回收率:
The recovery of human PAWR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 90-102 EDTA plasma (n=4) 94 90-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1600 2.745 2.652 2.699 2.569 800 2.246 2.176 2.211 2.081 400 1.761 1.704 1.733 1.603 200 1.167 1.139 1.153 1.023 100 0.524 0.569 0.547 0.417 50 0.312 0.309 0.311 0.181 25 0.198 0.189 0.194 0.064 0 0.127 0.133 0.130 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
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基因功能參考文獻(xiàn):
- These findings suggest that PAR4 plays a potential tumor suppressor role in esophageal squamous cell carcinoma cells PMID: 30363984
- we determined that increased miR-17-3P level plays crucial role in CRC cells survival by targeting Par4, contributing to colorectal carcinogenesis. PMID: 29115593
- PAR4 is the target of mir-107 in colorectal cancer cells. PMID: 27938501
- we confirmed that the RASSF2-PAR-4 axis was mainly responsible for miR-7 functions in CAFs using bioinformatics methods. Overexpression of miR-7 in CAFs led to down-regulation of RASSF2, which dramatically decreased the secretion of PAR-4 from CAFs and then enhanced the proliferation and migration of the co-cultured cancer cells. PMID: 27901488
- we investigated in the present study the mechanisms regulating the accumulation of a 25kDa cleaved-Par-4 (cl-Par-4) fragment in ovarian and endometrial cancer cell lines PMID: 27175591
- Authors demonstrate that TRIM21 expression predicts survival in pancreatic cancer patients. This work highlights a novel mechanism of Par-4 regulation, and identifies a novel prognostic marker and potential therapeutic target for pancreatic cancer. PMID: 27830973
- Data suggest that PAR4 and P2Y12 heterodimer internalization/endocytosis is required for beta-arrestin-2 recruitment to endosomes and up-regulation of Akt signaling; activation of PAR4 but not of P2Y12 drives internalization of the PAR4-P2Y12 heterodimer. (PAR4 = protease-activated receptor 4; P2Y12 = purinergic receptor P2Y, G-protein coupled, 12 protein; Akt = proto-oncogene protein c-akt) PMID: 28652403
- In this review, we will focus on the therapeutic perspective of Par-4 with a special reference to its (Par-4) virgin prospect of devastating metastasis control. PMID: 27568374
- in vitro and in vivo upregulation of Par-4 expression is indispensable for the trafficking of GRP78 to the cell membrane and subsequent apoptosis of cancer cell PMID: 28720068
- Prostate apoptosis response 4 (PAR4) expression modulates WNT signaling pathways in MCF7 breast cancer cells: A possible mechanism underlying PAR4-mediated docetaxel chemosensitivity. PMID: 28259909
- Decreased PAR4 expression in breast cancer is associated with shorter survival. PAR4 suppresses growth and invasiveness of breast cancer cells. PMID: 26977019
- Authors determined that PAR-4 induces cell apoptosis in response to stimuli, in vitro, but is also involved in the relocation of GRP78 from endoplasmic reticulum to the cell surface of ovarian cancer cell line. PMID: 26246468
- These results suggest that Porphyromonas gingivalis activates PAR4 signaling pathways, leading proMMP9 over-expression and cellular invasion in oral squamous cell carcinoma cells. PMID: 25670650
- PAR1-platelet releasate enhances vasculogenesis more potently than PAR4-platelet releasate, and the enhancements require a cooperation of multiple platelet-derived angiogenic regulators. PMID: 25495701
- A novel long non-coding RNA T-ALL-R-LncR1 knockdown and Par-4 cooperate to induce cellular apoptosis in T-cell acute lymphoblastic leukemia cells. PMID: 23906015
- Data indicate that PAR1 and PAR4 activate common promigratory signalling pathways in Hep3B liver carcinoma cells including activation of the receptor tyrosine kinases Met and PDGFR, the formation of ROS and the inactivation of PTP1B. PMID: 25373316
- a Par-4 mutant that is unable to bind Fbxo45 is stabilized and further enhances staurosporine-induced apoptosis. PMID: 24992930
- C-terminus of the rat homologue of Par-4 was crystallized and a 3.7 A resolution X-ray diffraction data set was collected PMID: 25195896
- These results indicate that the expression of PAR1 and PAR2 in esophageal squamous cell carcinoma is increased but PAR4 is decreased. PMID: 25297082
- our results indicate that the mechanism by which PAR-4 orchestrates the apoptotic process requires cleavage by caspase-8. PMID: 24931006
- Par-4 is expressed in trophoblastic cells and is involved in transport of GRP78 to the cell surface. PMID: 24282526
- The addition of TRAIL to WIN 55.212-2-treated cells led to apoptotic death probably mediated by up-regulation of the tumor suppressor factor PAR-4, whose levels increased after WIN treatment, and by the translocation of GRP78 on cell surface. PMID: 24795528
- The cancer cell specific activity of Par-4 is elicited through intracellular as well as extracellular mechanisms. PMID: 25001535
- prostate apoptosis response-4 (Par-4) has a role in human glioma stem cells in drug-induced apoptosis PMID: 24523904
- These results suggested a prognostic role of Par-4 in hypopharyngeal carcinoma. PMID: 24418097
- Phosphorylation by CK2 impairs Par-4 proapoptotic functions. PMID: 24457960
- Par-4 is a target of TGF-beta signaling and acts as an important factor during TGF-beta-induced epithelial-to-mesenchymal transition. PMID: 24503536
- Par-4 expression modulates apoptosis in response to docetaxel in MCF7 breast cancer cells. PMID: 23760770
- Down-regulation of protease-activated receptor 4 in lung adenocarcinoma is associated with a more aggressive phenotype PMID: 23886184
- Par-4-induced multinucleation as a mechanism of cell death in oncogene-addicted cells and establish Par-4 as a negative regulator of breast cancer recurrence. PMID: 23770012
- our results identify a novel intracellular pathway of apoptosis mediated by NF-kappaB through UACA elevation, which by attenuating endoplasmic reticulum stress and GRP78 translocation to the cell surface can blunt the sensitivity of cancer cells to apoptosis. PMID: 23204231
- Gamma-tocotrienol inhibited IL-13/STAT6-activated eotaxin secretion via up-regulation of PAR4 expression and enhancement of aPKC-PAR4 complex formation. PMID: 21764283
- a novel mechanism of apoptosis induction by PAR-4/ceramide-enriched exosomes, which may critically contribute to Alzheimer disease. PMID: 22532571
- identified a novel specific caspase-3 cleavage site in Par-4, and the cleaved fragment of Par-4 retains proapoptotic activity PMID: 22184067
- The biological significance of PrPc association with par-4 provided the first evidence of a relationship between the endogenous levels of PrPc and the resistance of glioma cells to the apoptotic effects of TMZ. PMID: 21328340
- 17beta-estradiol and Insulin-like growth factor-1 inhibit PAR-4 gene expression in MCF-7 cells. PMID: 21567071
- Par-4-overexpressing tumors exhibited a bystander effect on wild-type tumors growing distally in the same mouse. PMID: 21555373
- The expression of PAR-4 protein in B cells correlated positively with the percentage of CD38(+) cells, as well as with CD38(+)/ZAP-70(+) cells. PMID: 21526495
- Decreased Par-4 expression is associated with cholangiocarcinoma. PMID: 20724592
- siRNA against Par-4 gene could inhibit the apoptosis of human bone marrow mesenchymal stem cells. PMID: 19099901
- findings suggest that a lower expression level of Par-4 is related to an unfavorable prognosis in breast cancer patients PMID: 20637369
- Downregulation of PAR4 is associated with poor prognosis in breast cancer. PMID: 20514395
- Compared to normal controls, mean Par-4 levels appeared slightly lower in schizophrenia and bipolar disorder. However, in major depression, Par-4 was decreased by 67% compared to normal controls. PMID: 20067857
- Data show that RASSF2 forms a direct and endogenous complex with prostate apoptosis response protein 4 (PAR-4) and that this interaction is regulated by K-Ras and is essential for the full apoptotic effects of PAR-4. PMID: 20368356
- Endoplasmic reticulum stress causes translocation of the Par-4-GRP78 complex from the ER to the plasma membrane, and through a positive feedback loop, extracellular Par-4 binds to cell surface GRP78 activating the extrinsic apoptotic pathway. Review. PMID: 19823030
- Results suggest that, in breast cancer, Par-4 plays a similar tumor suppressor gene role as reported in endometrial carcinoma, and that Par-4 expression has a significant inverse association with expression of progesterone receptor. PMID: 20082875
- These data suggest PAWR is a novel PITX2-interacting protein that regulates PITX2 activity in ocular cells. PMID: 19801652
- mechanical strain increased PAR-4 gene expression in macrophages PMID: 11910304
- role in regulating Bcl-2 through a WT1-binding site on the bcl-2 promoter PMID: 12644474
- Par-4 enables cells to circumvent inhibition of the central executioner caspase-3 by alternative activation of caspases following a decrease in expression levels of inhibitors of apoptosis proteins PMID: 12685825
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亞細(xì)胞定位:Cytoplasm. Nucleus.
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組織特異性:Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
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