Human Pro-Nerve growth factor(proNGF)ELISA kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人神經(jīng)生長因子前體(proNGF)酶聯(lián)免疫試劑盒（CSB-E17311h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的proNGF含量。proNGF 即前神經(jīng)生長因子，它是神經(jīng)生長因子的前體形式。相關(guān)研究顯示，proNGF 可能參與神經(jīng)系統(tǒng)發(fā)育、損傷修復(fù)等過程，其異常表達與神經(jīng)退行性疾病等發(fā)病機制有關(guān)，在細胞凋亡調(diào)控等方面有著重要作用，是神經(jīng)領(lǐng)域研究的一個重要靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外研究神經(jīng)退行性疾病模型中的生物標(biāo)志物表達、藥物干預(yù)效果評估或相關(guān)信號通路機制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

proNGF
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human proNGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	93
	Range %	89-96
1:4	Average %	90
	Range %	85-96
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of human proNGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.