Mouse Pro-Nerve growth factor (proNGF) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠神經(jīng)生長因子前體(proNGF)酶聯(lián)免疫試劑盒（CSB-EQ027721MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的proNGF含量。小鼠神經(jīng)生長因子前體（proNGF）是神經(jīng)生長因子（NGF）的前體形式，參與神經(jīng)元發(fā)育、存活和突觸形成。其研究機(jī)制涉及與受體結(jié)合、信號(hào)傳導(dǎo)以及與疾病如抑郁癥和乳腺癌轉(zhuǎn)移的相關(guān)性。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于神經(jīng)生物學(xué)基礎(chǔ)研究、疾病動(dòng)物模型評(píng)估及藥物干預(yù)實(shí)驗(yàn)中proNGF表達(dá)水平的動(dòng)態(tài)監(jiān)測，尤其可為帕金森模型、脊髓損傷模型等研究提供定量分析工具，為探索神經(jīng)發(fā)育機(jī)制及退行性病變提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

proNGF
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse proNGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	102
	Range %	97-106
1:4	Average %	85
	Range %	81-89
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of mouse proNGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-106
EDTA plasma (n=4)	89	84-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.