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  4. Human Retinoic Acid ELISA Kit
  • 中文名稱(chēng)：
    人視黃酸酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E16712h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人視黃酸酶聯(lián)免疫試劑盒（CSB-E16712h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)樣本中的Retinoic Acid含量。Retinoic Acid（視黃酸）是維生素A的衍生物，在胚胎發(fā)育、細(xì)胞分化等生理過(guò)程中起關(guān)鍵作用。研究機(jī)制上，它通過(guò)與核受體結(jié)合，調(diào)控基因表達(dá)，影響細(xì)胞生長(zhǎng)、分化和凋亡。在腫瘤治療等領(lǐng)域，視黃酸具有潛在應(yīng)用前景?？蒲腥藛T可借助開(kāi)展視黃酸代謝調(diào)控研究、疾病模型中的分子機(jī)制探索，或評(píng)估藥物干預(yù)對(duì)視黃酸信號(hào)通路的影響，為發(fā)育生物學(xué)、腫瘤微環(huán)境及免疫相關(guān)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    Retinoic Acid
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
  • 檢測(cè)范圍：
  • 靈敏度：
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線(xiàn)性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    93

    Range %

    86-100

    1:200

    Average %

    88

    Range %

    81-95

    1:400

    Average %

    96

    Range %

    89-102

    1:800

    Average %

    85

    Range %

    81-90

  • 回收率：

    The recovery of human retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    83-97

    EDTA plasma (n=4)

    87

    82-94

  • 標(biāo)準(zhǔn)曲線(xiàn)：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    100

    0.146

    0.138

    0.142

    50

    0.325

    0.337

    0.331

    25

    0.649

    0.618

    0.634

    12.5

    1.123

    1.059

    1.091

    6.25

    1.685

    1.601

    1.643

    0

    2.096

    2.103

    2.100

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Per customers request, please provide the following information regarding Human Retinoic Acid ELISA Kit (CSB-E16712h)
a. What were the antibodies used in this kit raised against?
b. What is the standard? (Recombinant full-length protein? Partial protein
c. (range?)?, peptide (sequence range?), native protein?)
e. If recombinant protein, then what is the host?
f. Is the capture antibody a monoclonal or polyclonal?
g. What is the host of the capture antibody?
h. What is the immunogen of the capture antibody?
i. Is the detection antibody a monoclonal or polyclonal?
j. What is the host of the detection antibody?
k. What is the immunogen of the detection antibody?
l. Can this assay be used with another species besides human?

A:
Thanks for your inquiry! CSB-E16712h This kit is based on competitive method. Retinoic Acid is small molecule.The plate is coated with specific antibody for Retinoic Acid. It is mouse monoclonal antibody and immunogen is modified by special modification. Sorry we can not disclose how it is modified. The antigen in the sample and HRP conjugated antigen compete to bind with the specific antibody. Standard is purified Retinoic Acid small molecule.
Since for different species the sample matrix is different, we have developed Retinoic Acid elisa kits for different species. We suggest you choose the corresponding kit so as to avoid matrix interference.