搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse Retinoic Acid ELISA kit

Mouse Retinoic Acid ELISA kit

  • 中文名稱：
    小鼠視黃酸酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ028019MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠視黃酸酶聯(lián)免疫試劑盒（CSB-EQ028019MO）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的Retinoic Acid含量。Retinoic Acid是一種視黃酸，在生物體發(fā)育、細(xì)胞分化等過程中扮演重要角色。其研究機(jī)制主要圍繞與核內(nèi)受體結(jié)合，調(diào)控基因表達(dá)，影響細(xì)胞增殖、分化和凋亡等生物學(xué)行為，在腫瘤治療、皮膚疾病等領(lǐng)域具有潛在應(yīng)用價(jià)值。試劑盒檢測范圍為25 pg/mL-400 pg/mL，該產(chǎn)品可廣泛應(yīng)用于評(píng)估藥物或營養(yǎng)干預(yù)對(duì)視黃酸代謝通路的影響、構(gòu)建疾病模型（如代謝異?；虬l(fā)育缺陷模型）時(shí)的生物標(biāo)志物檢測，以及基礎(chǔ)研究中視黃酸信號(hào)通路的動(dòng)態(tài)變化研究，為科研人員提供穩(wěn)定可靠的體外檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Retinoic Acid
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    25 pg/mL-400 pg/mL
  • 靈敏度：
    12.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 92  
    Range % 87-97  
    1:40 Average % 90  
    Range % 85-94  
    1:80 Average % 84  
    Range % 80-88  
    1:160 Average % 89  
    Range % 84-93  
  • 回收率：
    The recovery of mouse retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 92-103  
    EDTA plasma (n=4) 94 90-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.113 0.110 0.112    
    200 0.257 0.246 0.252    
    100 0.451 0.437 0.444    
    50 0.709 0.726 0.718    
    25 1.103 1.081 1.092    
    0 1.672 1.629 1.651    
  • 本試劑盒所含材料：
    • An assay plate --- The 96-well plate has been pre-coated with the anti-mouse retinoic acid antibody.
    • Lyophilized standard (1 x 200 μl) --- Reconstitute the standard with sample diluent at dilution series and draw the standard curve.
    • HRP-conjugate retinoic acid (100 x concentrate) (1 x 60 μl)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
    • HRP-avidin Diluent (1 x 10 ml) --- Dilute the HRP-avidin.
    • Sample Diluent (2 x 20 ml) --- Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25 x concentrate) (1 x 20 ml) --- Wash away the unbound solution and non-specific substances.
    • TMB Substrate --- TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microtiter plate when incubation.
    • An instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

    顯示更多

    收起更多

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)產(chǎn)品