-
中文名稱:人硬骨素(SOST)酶聯(lián)免疫試劑盒
-
貨號:CSB-E13146h
-
規(guī)格:96T/48T
-
價格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:人硬骨素(SOST)酶聯(lián)免疫試劑盒(CSB-E13146h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SOST含量。SOST是重要靶點。它是一種骨硬化蛋白,在骨代謝中起關(guān)鍵作用。其可抑制成骨細(xì)胞活性,影響骨形成。目前關(guān)于它的研究聚焦于通過抑制SOST來促進骨生長,對治療骨質(zhì)疏松等骨相關(guān)疾病有潛在價值。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,該產(chǎn)品廣泛應(yīng)用于骨代謝疾病基礎(chǔ)研究、抗骨質(zhì)疏松藥物開發(fā)中的生物標(biāo)志物檢測、骨組織工程材料的體外效能評估,以及動物模型中骨形成過程的動態(tài)監(jiān)測本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名:BEER ELISA Kit; CDD ELISA Kit; Cortical hyperostosis with syndactyly ELISA Kit; Sclerosteosis ELISA Kit; Sclerostin ELISA Kit; Sost ELISA Kit; SOST_HUMAN ELISA Kit; SOST1 ELISA Kit; UNQ2976/PRO7455/PRO7476 ELISA Kit; VBCH ELISA Kit
-
縮寫:
-
Uniprot No.:
-
種屬:Homo sapiens (Human)
-
樣本類型:serum, plasma, tissue homogenates
-
檢測范圍:31.25 pg/mL-2000 pg/mL
-
靈敏度:7.8 pg/mL
-
反應(yīng)時間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領(lǐng)域:Signal Transduction
-
測定原理:quantitative
-
測定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 80-92 1:2 Average % 95 Range % 90-100 1:4 Average % 96 Range % 90-101 1:8 Average % 93 Range % 88-99 -
回收率:
The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 89-99 EDTA plasma (n=4) 90 85-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 1.932 1.904 1.918 1.808 1000 1.516 1.481 1.499 1.389 500 1.112 1.101 1.107 0.997 250 0.733 0.687 0.710 0.600 125 0.547 0.504 0.526 0.416 62.5 0.329 0.309 0.319 0.209 31.25 0.197 0.181 0.189 0.079 0 0.112 0.107 0.110 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
引用文獻
- Association Between Plasma Sclerostin Levels and Body Mass Index in Women With Polycystic Ovary Syndrome M Bertizlioglu,Archives of gynecology and obstetrics,2023
- Comparative Analysis of Vascular Calcification Risk Factors in Pre-hemodialysis and Prevalent Hemodialysis Adult Patients: Insights into Calcification Biomarker Associations and Implications for Intervention Strategies in Chronic Kidney Disease X Lan,/,2024
- Role of sclerostin and dkk1 in bone remodeling in type 2 diabetic patients Wang N.et al,Endocr Res.,2017
- Serum sclerostin values are associated with abdominal aortic calcification and predict cardiovascular events in patients with chronic kidney disease stages 3-5D. Wang XR.et al,Nephrology (Carlton).,2016
- The effect of single extremity-vibration on the serum sclerostin level Halil Ibrahim Cakar. et al,Journal of Physical Therapy Science,2015
- Paeoniflorin Atttenuates Amyloidogenesis and the Inflammatory Responses in a Transgenic Mouse Model of Alzheimer´s Disease Hong-Ri Zhang. et al,Neurochemical Research,2015
- Serum YKL-40/chitinase 3-like protein 1 level is an independent predictor of atherosclerosis development in patients with obstructive sleep apnoea syndrome Eftal Murat Bakirci. et al,Turk Kardiyol Dern Ars,2015
- Effects of sex steroids on serum sclerostin levels during the menstrual cycle. Cidem M et al,Gynecol Obstet Invest,2013
相關(guān)產(chǎn)品
靶點詳情
-
功能:Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
-
基因功能參考文獻:
- Sclerostin is degraded by cathepsin K in vitro. Cathepsin K degradation of sclerostin is affected by hypoxia. PMID: 29859187
- Study found that sclerostin concentrations were not significantly higher in patients with bone metastases compared to non-metastatic prostate cancer (PC) but they were significantly higher in patients with CRPC. Sclerostin levels were significantly higher in patients with advanced disease and increased bone turnover due to a compensatory response to the increased number of osteoblasts. PMID: 30116403
- Patients with Latent autoimmune diabetes in Adults (LADA) presented lower bone resorption than did controls, similar to patients with T2 diabetes (T2D). Sclerostin is increased in T2D but not in LADA, suggesting possible roles on bone metabolism in T2D only. PMID: 29506222
- Higher Sclerostin/SOST expression is associated with lower percentage of circulatory blasts and better prognosis in patients with myelofibrosis. PMID: 29532161
- Sclerosteosis is caused by loss-of-function mutations in the SOST gene which encodes a secreted glycoprotein, sclerostin--{REVIEW} PMID: 29080811
- Serum sclerostin level was not an independent predictor of mortality in Maintenance Hemodialysis Patients PMID: 29940587
- these results suggest a possible role of sclerostin in the identification of ankylosing spondylitis patients PMID: 29854850
- important role for SOST SNP rs1877632 and VDR SNPs rs10735810 and rs731236 in the pathophysiology of stress fracture PMID: 29129460
- Sclerostin increased after exercise in comparison to baseline (mean +/- SEM: 410 +/- 27 vs. 290 +/- 19 pg/mL; p < 0.001) corresponding to an increase of +44.3 +/-5.5% PMID: 28374174
- serum sclerostin levels correlated positively with carotid intima-media thickness and inversely with the augmentation index, a marker of arterial stiffness PMID: 28339088
- The difference of serum sclerostin levels in Ankylosing Spondylitis and Rheumatoid Arthritis patients was not significantly different from HC, indicating that the sclerostin may not associate with the development of Ankylosing Spondylitis and Rheumatoid Arthritis. PMID: 28553652
- SOST gene silencing promotes the proliferation, invasion, and migration, and inhibits apoptosis of osteosarcoma cells by activating Wnt/beta-catenin signaling pathway PMID: 28246931
- No difference was found in the serum sclerostin levels between the hyperthyroidism patients and healthy control. PMID: 29059259
- Positivity of RANKL and anti-CCP2 yielded significant risk for progression with negativity for both as reference. No single nucleotide polymorphism encoding TNFSF11 or SOST was associated with increased concentrations of the factors. PMID: 28190118
- Osterix and RUNX2 are transcriptional regulators of sclerostin in human bone PMID: 27154028
- An association was found between rs851054 of the SOST promoter and the fracture rate during childhood osteogenesis imperfecta. PMID: 27519970
- The increased expression of sclerostin in the liver and the association with histologic cholangitis may explain the high serum levels of this protein in patients with primary biliary cirrhosis. PMID: 27019303
- SOST silencing promotes the proliferation, invasion and migration, and decreases the apoptosis of human retinoblastoma cells by activating the Wnt/beta-catenin signaling pathway. PMID: 28485721
- Sclerostin concentrations in serum significantly decreased and IGF-I significantly increased after 12months of resistance training or JUMP. PMID: 27744012
- observed an association between sclerostin levels with fasting insulin levels and homoeostatic model assessment-insulin resistance, but there was no clear association with type 2 diabetes risk. PMID: 28090669
- Sclerostin levels in KTR are normal and influenced more by bone turnover than by eGFR. Its involvement with other hormones of mineral homeostasis (FGF23/Klotho and Vitamin D) is part of the sophisticated cross-talk between bone and the kidney PMID: 28558021
- Vitamin D receptor agonism by paricalcitol causes a moderate increase in serum sclerostin in CKD patients, and this effect is modified by circulating pentosidine levels. PMID: 28017524
- SOST is frequently expressed in skeletal bone- and cartilage-forming tumors. The strong spatial correlation with bone formation and the in vitro expression patterns are in line with the known functions of SOST in nonneoplastic bone, as a feedback inhibitor on osteogenic differentiation. PMID: 27498059
- Intermittent compressive stress regulates Notch receptor and target gene expression via the TGF-beta signaling pathway. Notch signaling participates in TGF-beta-induced sclerostin expression in periodontal ligament cells. PMID: 27966788
- Dickkopf-1 and sclerostin were never correlated with each other or with bone turnover markers patients with Paget's disease of bone. Sclerostin was positively correlated with age. PMID: 28054306
- These data suggest that sclerostin plays an important role in the bone remodeling of tooth movement. PMID: 28081119
- SOST is expressed in the aorta and downregulated in human aortic aneurysms and atherosclerosis, possibly because of epigenetic silencing. PMID: 28062506
- The level of sclerostin was higher in the female obstructive sleep apnea (OSA) patients than that in female controls. Further, in OSA women with cardiovascular comorbidities, sclerostin was higher than in women without such comorbidities. In men, there were no differences in the serum sclerostin level between the OSA and control subjects, nor was there any relationship with cardiovascular diseases. PMID: 26820731
- Knockdown of SOST in MG-63 cells increases osteogenesis and ratio of OPG/RANKL in vitro PMID: 27774939
- The findings confirm that the human SOST gene and sclerostin expression can be considered to be directly 1,25-dihydroxyvitamin D-responsive in osteocytes. PMID: 26690786
- Sclerostin is an osteocyte marker that is strongly expressed in human woven and lamellar bone and mineralizing chondrocytes PMID: 26896083
- similar levels in type 1 diabetes patients and controls; decrease concurrent with adolescent growth spurt PMID: 26094958
- Findings indicate that sclerostin expression is closely associated with the degree of joint damage in primary knee osteoarthritis (OA), confirming its involvement in the development of OA. PMID: 27665782
- Results suggest that sclerostin may have a role in the development of or the response to abdominal aortic calcification in chronic kidney disease. PMID: 27165564
- serum sclerostin levels were lower in nonalcoholic steatohepatitis patients than in controls. PMID: 26056025
- The aim of this study was to evaluate the renal handling of sclerostin. PMID: 27214309
- chronic TNFalpha (tumor necrosis factor alpha)-dependent arthritis, fibroblast-like synoviocytes constitute a major source of sclerostin and that either the lack of sclerostin or its antibody-mediated inhibition leads to an acceleration of rheumatoid arthritis (RA)-like disease. PMID: 27089204
- Sclerostin levels are elevated in CKD patients and are associated with inflammation, vascular lesions, uremia and (potentially) mortality. PMID: 25070604
- Our data also demonstrated that vitamin D deficient newborns exhibited lower sclerostin levels than vitamin D sufficient newborns. PMID: 26352089
- the first evidence suggesting that LRP4 is responsible for the retention of sclerostin in the bone environment in humans. PMID: 26751728
- higher serum sclerostin levels are associated with higher bone mineral density, lower aortic calcification scores, and a better survival rate in hemodialysis patients PMID: 26890570
- findings suggest that serum TGF-beta1 level increases during postmenopause and declines in old age. Sclerostin production is inhibited by TGF-beta1 during early postmenopause PMID: 26826396
- a cell model of human dermal fibroblasts in order to investigate the functions of sclerostin, is reported. PMID: 26851122
- The aims of this study were to evaluate OCN and sclerostin levels in subjects who underwent coronary artery bypass graft (CABG) surgery compared with those in normal controls. PMID: 26576474
- two affected siblings who carried a novel nonsense mutation of SOST in a consanguineous family from China, is reported. PMID: 26283468
- Sclerostin serum levels are not associated with an adverse metabolic profile during pregnancy in women with GDM and PE PMID: 25753744
- Quantitative RT-PCR analysis showed that SOST expression dose-dependently decreased with increasing Wnt signaling, while BMP4 induced SOST expression PMID: 26095393
- The correlation of SOST polymorphisms with changes of BMD and bone biomarkers after treatment was analyzed. PMID: 26250343
- highly sulfated glycosaminoglycans might control bone homeostasis via interference with sclerostin/LRP5/6 complex formation. PMID: 26232882
- Serum sclerostin was associated with serum intact parathyroid hormone in patients undergoing peritoneal dialysis. PMID: 25974190
顯示更多
收起更多
-
相關(guān)疾?。?/div>Sclerosteosis 1 (SOST1); Van Buchem disease (VBCH); Craniodiaphyseal dysplasia autosomal dominant (CDD)亞細(xì)胞定位:Secreted, extracellular space, extracellular matrix.蛋白家族:Sclerostin family組織特異性:Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level).數(shù)據(jù)庫鏈接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-