Human Semenogelin-2(SEMG2) ELISA kit
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中文名稱:人精囊蛋白-2(SEMG2)酶聯(lián)免疫試劑盒
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貨號:CSB-EL021002HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人精囊蛋白-2(SEMG2)酶聯(lián)免疫試劑盒(CSB-EL021002HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SEMG2含量。人精囊蛋白-2(SEMG2)是一種由精囊腺分泌的蛋白質(zhì),主要功能是形成精子周圍的凝膠狀物質(zhì),限制精子運(yùn)動。SEMG2在靈長動物的性別選擇行為中起關(guān)鍵作用,其基因的進(jìn)化速度受性別選擇行為影響,進(jìn)而影響求偶時(shí)的競爭優(yōu)勢。此外,SEMG2在腫瘤免疫逃逸中也發(fā)揮作用,其與多種免疫受體結(jié)合,抑制T細(xì)胞對腫瘤細(xì)胞的殺傷。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,本產(chǎn)品適用于科研領(lǐng)域中對精液蛋白功能、男性生殖生理機(jī)制的研究,或通過檢測生物樣本中SEMG2水平探索其與生殖系統(tǒng)疾病的潛在關(guān)聯(lián),為體外實(shí)驗(yàn)提供高特異性的定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:SEMG2 ELISA Kit; Semenogelin-2 ELISA Kit; Semenogelin II ELISA Kit; SGII ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.81 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SEMG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 85-94 1:2 Average % 92 Range % Sep-87 1:4 Average % 92 Range % 85-96 1:8 Average % 89 Range % 87-92 -
回收率:
The recovery of human SEMG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 86-96 EDTA plasma (n=4) 103 98-107 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.423 2.579 2.501 2.393 1000 1.954 2.054 2.004 1.896 500 1.265 1.302 1.284 1.176 250 0.712 0.734 0.723 0.615 125 0.416 0.405 0.411 0.303 62.5 0.251 0.266 0.259 0.151 31.25 0.176 0.183 0.180 0.072 0 0.107 0.108 0.108 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
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基因功能參考文獻(xiàn):
- SgII transcripts were demonstrated in several tissues, with the strongest signals coming from seminal vesicles, vas deferens, prostate, epididymis and trachea. PMID: 12200457
- SGII is a novel target for protein S-nitrosylation in spermatozoa. PMID: 17683036
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亞細(xì)胞定位:Secreted.
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蛋白家族:Semenogelin family
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組織特異性:Seminal vesicles, and to a much lesser extent, epididymis.
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數(shù)據(jù)庫鏈接:
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