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  4. Human Sialic acid-binding Ig-like lectin 8(SIGLEC8) ELISA kit

Human Sialic acid-binding Ig-like lectin 8(SIGLEC8) ELISA kit

  • 中文名稱：
    人唾液酸結(jié)合Ig樣凝集素8(SIGLEC8)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL021302HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人唾液酸結(jié)合Ig樣凝集素8(SIGLEC8)酶聯(lián)免疫試劑盒（CSB-EL021302HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SIGLEC8含量。SIGLEC8是一個(gè)重要靶點(diǎn)。它主要表達(dá)于嗜酸性粒細(xì)胞等免疫細(xì)胞表面，在調(diào)節(jié)免疫反應(yīng)中發(fā)揮作用。研究表明，針對(duì)SIGLEC8靶向治療有望用于過(guò)敏、哮喘等疾病，通過(guò)阻斷相關(guān)信號(hào)通路來(lái)抑制免疫細(xì)胞活性，從而改善疾病癥狀。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品適用于基礎(chǔ)研究中探索SIGLEC8在呼吸道疾病、自身免疫疾病中的分子機(jī)制，或用于體外藥物篩選模型中評(píng)估靶向治療藥物對(duì)SIGLEC8通路的影響，為免疫調(diào)節(jié)研究及生物標(biāo)志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    CD329 antigen ELISA Kit; CDw329 ELISA Kit; SAF 2 ELISA Kit; SAF-2 ELISA Kit; SAF2 ELISA Kit; Sialic acid binding Ig like lectin 8 ELISA Kit; Sialic acid-binding Ig-like lectin 8 ELISA Kit; Sialoadhesin family member 2 ELISA Kit; SIGL8_HUMAN ELISA Kit; Siglec 8 ELISA Kit; Siglec-8 ELISA Kit; SIGLEC8 ELISA Kit; SIGLEC8L ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 100
    Range % 95-107
    1:2 Average % 96
    Range % 92-100
    1:4 Average % 84
    Range % 80-92
    1:8 Average % 88
    Range % 84-92
  • 回收率：
    The recovery of human SIGLEC8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-100
    EDTA plasma (n=4) 90 85-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.164 2.246 2.205 2.040
    5 1.618 1.634 1.626 1.461
    2.5 1.201 1.175 1.188 1.023
    1.25 0.792 0.823 0.808 0.643
    0.625 0.558 0.571 0.565 0.400
    0.312 0.419 0.442 0.431 0.266
    0.156 0.324 0.305 0.315 0.150
    0 0.162 0.167 0.165 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
  • 基因功能參考文獻(xiàn)：
    1. In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
    2. miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
    3. Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
    4. Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
    5. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
    6. Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
    7. Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
    8. Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
    9. expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
    10. The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
    11. Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
    12. Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
    13. Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
    14. IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
    15. First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
    16. A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
    17. REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
    18. Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
  • 組織特異性：
    Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 10877

    OMIM: 605639

    KEGG: hsa:27181

    STRING: 9606.ENSP00000321077

    UniGene: Hs.447899