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SIGLEC8 Recombinant Monoclonal Antibody

  • 中文名稱：
    SIGLEC8 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA878931MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA878931MA1HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    SIGLEC8 Recombinant Monoclonal Antibody（CSB-RA878931MA1HU）是一款高特異性抗體，靶向唾液酸結(jié)合免疫球蛋白型凝集素8（SIGLEC8），該蛋白屬于免疫調(diào)節(jié)受體家族，主要在嗜酸性粒細(xì)胞和肥大細(xì)胞中表達(dá)，參與調(diào)控炎癥反應(yīng)及免疫細(xì)胞活化等生物學(xué)過程。本產(chǎn)品通過親和純化工藝制備，經(jīng)嚴(yán)格的質(zhì)控驗(yàn)證，確認(rèn)其在ELISA和免疫組化（IHC）實(shí)驗(yàn)中具有優(yōu)異的性能，其中IHC推薦使用稀釋比為1:50-1:200。實(shí)驗(yàn)數(shù)據(jù)顯示，該抗體在石蠟包埋組織切片中可清晰標(biāo)記目標(biāo)蛋白，定位準(zhǔn)確且背景信號(hào)低，適用于檢測(cè)人源組織樣本中SIGLEC8的表達(dá)分布。憑借其高靈敏度和批次穩(wěn)定性，該抗體可作為研究過敏性疾病、哮喘、慢性炎癥及免疫細(xì)胞功能調(diào)控機(jī)制的可靠工具，為探索相關(guān)疾病的分子機(jī)制提供技術(shù)支持。
  • Uniprot No.：
  • 基因名：
  • 別名：
    CD329 antigen antibody; CDw329 antibody; SAF 2 antibody; SAF-2 antibody; SAF2 antibody; Sialic acid binding Ig like lectin 8 antibody; Sialic acid-binding Ig-like lectin 8 antibody; Sialoadhesin family member 2 antibody; SIGL8_HUMAN antibody; Siglec 8 antibody; Siglec-8 antibody; SIGLEC8 antibody; SIGLEC8L antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human SIGLEC8 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    hIgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    18D13
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
  • 基因功能參考文獻(xiàn)：
    1. In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
    2. miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
    3. Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
    4. Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
    5. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
    6. Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
    7. Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
    8. Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
    9. expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
    10. The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
    11. Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
    12. Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
    13. Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
    14. IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
    15. First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
    16. A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
    17. REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
    18. Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
  • 組織特異性：
    Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 10877

    OMIM: 605639

    KEGG: hsa:27181

    STRING: 9606.ENSP00000321077

    UniGene: Hs.447899