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  4. Human activated protein C,APC ELISA Kit

Human activated protein C,APC ELISA Kit

  • 中文名稱：
    人活化蛋白C(APC)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09909h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人活化蛋白C(APC)酶聯(lián)免疫試劑盒（CSB-E09909h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APC含量。APC（腺瘤性結(jié)腸息肉病基因）是一種腫瘤抑制蛋白，作為Wnt信號(hào)傳導(dǎo)途徑的拮抗劑，其突變與家族性腺瘤性息肉?。‵AP）和結(jié)直腸癌的發(fā)生密切相關(guān)。研究APC機(jī)制主要關(guān)注其如何通過(guò)調(diào)節(jié)Wnt通路、細(xì)胞遷移、粘附、轉(zhuǎn)錄激活和細(xì)胞凋亡等過(guò)程，影響腫瘤的發(fā)生和發(fā)展。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，適用于凝血調(diào)控機(jī)制、炎癥相關(guān)疾病模型或藥物干預(yù)等基礎(chǔ)研究；在血栓性疾病、敗血癥及腫瘤微環(huán)境等研究領(lǐng)域具有重要價(jià)值本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 87-96  
    1:200 Average % 94  
    Range % 90-97  
    1:400 Average % 97  
    Range % 94-103  
    1:800 Average % 94  
    Range % 90-98  
  • 回收率：
    The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-94  
    EDTA plasma (n=4) 87 84-92  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    100 2.755 2.990 2.873 2.754  
    50 2.065 2.201 2.133 2.014  
    25 1.359 1.288 1.324 1.205  
    12.5 0.853 0.816 0.835 0.716  
    6.25 0.470 0.473 0.472 0.353  
    3.12 0.325 0.302 0.314 0.195  
    1.56 0.184 0.192 0.188 0.069  
    0 0.115 0.122 0.119    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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