Pig activated protein C(APC) ELISA Kit
-
中文名稱:豬活化蛋白C(APC)酶聯(lián)免疫試劑盒
-
貨號(hào):CSB-E12633p
-
規(guī)格:96T/48T
-
價(jià)格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:豬活化蛋白C(APC)酶聯(lián)免疫試劑盒(CSB-E12633p)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的APC含量。APC(腺瘤性息肉病基因)是一種腫瘤抑制基因,其突變與結(jié)直腸癌等癌癥的發(fā)生密切相關(guān)。研究機(jī)制顯示,APC基因突變會(huì)導(dǎo)致β-catenin蛋白積累,進(jìn)而激活下游原癌基因,促進(jìn)腫瘤形成。通過研究APC基因及其相關(guān)信號(hào)通路,有助于開發(fā)新的癌癥治療策略。試劑盒檢測(cè)范圍為40 pg/mL-8000 pg/mL,該產(chǎn)品適用于科研領(lǐng)域,如豬凝血機(jī)制研究、炎癥相關(guān)疾病模型(如敗血癥或血栓性疾病)的建立與評(píng)估,以及抗凝藥物或生物制劑開發(fā)過程中藥效動(dòng)力學(xué)研究。其兼容多種樣本類型的特點(diǎn),可支持不同實(shí)驗(yàn)設(shè)計(jì)需求,例如通過組織勻漿分析特定器官(如肝臟或血管內(nèi)皮)中APC的局部表達(dá)水平,為深入探究APC在病理生理過程中的調(diào)控機(jī)制提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名:N/A
-
縮寫:
-
種屬:Sus scrofa (Pig)
-
樣本類型:serum, plasma, tissue homogenates
-
檢測(cè)范圍:40 pg/mL-8000 pg/mL
-
靈敏度:20 pg/mL
-
反應(yīng)時(shí)間:1-5h
-
樣本體積:50-100ul
-
檢測(cè)波長(zhǎng):450 nm
-
研究領(lǐng)域:Signal Transduction
-
測(cè)定原理:quantitative
-
測(cè)定方法:Competitive
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 85-99 1:2 Average % 95 Range % 89-101 1:4 Average % 94 Range % 91-99 1:8 Average % 96 Range % 93-99 -
回收率:
The recovery of pig APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 97 91-104 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 0 2.437 2.458 2.448 40 1.462 1.482 1.472 160 1.005 1.018 1.012 640 0.608 0.617 0.613 2000 0.357 0.342 0.350 8000 0.200 0.204 0.202 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
相關(guān)產(chǎn)品
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-