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  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit

Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit

  • 中文名稱：
    人抗雙鏈DNA(dsDNA)抗體/天然DNA抗體(IgG)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04911h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人抗雙鏈DNA(dsDNA)抗體/天然DNA抗體(IgG)酶聯(lián)免疫試劑盒（CSB-E04911h）為間接法ELISA試劑盒，定性檢測(cè)血清樣本中的dsDNA Ab (IgG)含量。dsDNA Ab (IgG)即抗雙鏈 DNA 抗體（IgG 型），其產(chǎn)生與機(jī)體自身免疫紊亂有關(guān)，常見于系統(tǒng)性紅斑狼瘡等自身免疫病。研究認(rèn)為它可與體內(nèi)雙鏈 DNA 結(jié)合形成免疫復(fù)合物，沉積于組織引發(fā)炎癥反應(yīng)，干擾正常生理功能，是相關(guān)疾病重要研究靶點(diǎn)。本產(chǎn)品適用于科研場(chǎng)景中SLE相關(guān)抗體水平分析、疾病活動(dòng)度關(guān)聯(lián)研究，或用于評(píng)估實(shí)驗(yàn)性治療對(duì)自身免疫反應(yīng)的影響，為免疫病理學(xué)研究及藥物開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    dsDNA Ab (IgG)
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Autoimmunity
  • 測(cè)定原理：
    qualitative
  • 測(cè)定方法：
    Indirect
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標(biāo)準(zhǔn)曲線：

    Test parameter

    specification

    test result

    Positive control

    >0.6

    1.246

    Negative control

    <0.15

    0.053

    Positive rate

    10，Positive

    100%

    Negative rate

    10，Negative

    100%

  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with antigen-specific for dsDNA Ab (IgG). This dismountable microplate can be divided into 12 x 8 strip plates.
    • Negative Control (1 x 800 μl)
    • Positive Control (1 x 800 μl)
    • HRP-conjugate (1 x10 ml) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Sample Diluent (1 x 20 ml) --- Dilute the standard and sample solution.
    • Wash Buffer (25 x concentrate) (1 x20 ml) --- Wash away unbound or free substances.
    • Substrate A 1 x 5 ml --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Substrate B 1 x 5 ml --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Stop Solution (1 x 5 ml) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清 ,血漿（抗凝劑） EDTA

樣品信息：小鼠

稀釋比：其他 1:10

產(chǎn)品評(píng)價(jià)： 我用華美生物貨號(hào)為CSB-E04911h的ELISA kit檢測(cè)空白或人源化PBMC重建小鼠模型血清、正常人或炎癥患者中的anti-dsDNA的水平，樣本上樣前給予不同的稀釋倍數(shù)，再分析OD值，確認(rèn)cutoff 值和最終的樣本稀釋倍數(shù)。

By 張老師