Human apelin 12,AP12 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人apelin 12(AP12)酶聯(lián)免疫試劑盒（CSB-E08639h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、唾液、尿液、組織勻漿樣本中的AP12含量。人apelin 12（AP12）是一種多肽，對孤兒受體APJ具有高親和力，參與體液平衡調(diào)節(jié)和攝食中樞控制，阻斷HIV-1通過APJ受體進(jìn)入，并具有神經(jīng)保護(hù)作用。研究顯示，AP12在心血管疾病、神經(jīng)系統(tǒng)疾病等方面具有潛在應(yīng)用價值。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，特別適用于基礎(chǔ)醫(yī)學(xué)研究中AP12在代謝調(diào)控機(jī)制探索、心血管疾病模型建立及生物標(biāo)志物篩選等方向的應(yīng)用，為體外檢測不同生理或病理?xiàng)l件下AP12在體液或組織中的動態(tài)表達(dá)提供可靠工具，滿足科研人員對多類型樣本的并行分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AP12
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, urine, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AP12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-95
1:2	Average %	87
	Range %	84-90
1:4	Average %	84
	Range %	81-87
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of human AP12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	89	85-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.