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  4. Human asymmetrical dimethylarginine,ADMA ELISA Kit

Human asymmetrical dimethylarginine,ADMA ELISA Kit

  • 中文名稱：
    人不對稱二甲基精氨酸(ADMA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09298h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人不對稱二甲基精氨酸(ADMA)酶聯(lián)免疫試劑盒（CSB-E09298h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ADMA含量。ADMA即不對稱二甲基精氨酸，是內(nèi)源性一氧化氮合酶（NOS）的抑制劑。它可導(dǎo)致血管內(nèi)皮功能障礙，在心血管疾病、腎臟疾病等多種疾病發(fā)生發(fā)展中起重要作用。目前研究關(guān)注其產(chǎn)生、代謝機制及降低其水平以改善疾病預(yù)后的策略。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，本產(chǎn)品適用于心血管疾病模型構(gòu)建、代謝綜合征機制研究、腎臟功能調(diào)控實驗等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    ADMA
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %94-105
    1:2Average %94
    Range %90-98
    1:4Average %95
    Range %91-100
    1:8Average %93
    Range %89-97
  • 回收率：
    The recovery of human ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)8782-93
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.763 2.612 2.688 2.552
    2502.249 2.149 2.199 2.063
    1251.467 1.428 1.448 1.312
    62.50.883 0.823 0.853 0.717
    31.20.497 0.505 0.501 0.365
    15.60.357 0.386 0.372 0.236
    7.80.241 0.229 0.235 0.099
    00.139 0.132 0.136 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ADMA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled ADMA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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