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  4. Rat asymmetrical dimethylarginine(ADMA) ELISA Kit

Rat asymmetrical dimethylarginine(ADMA) ELISA Kit

  • 中文名稱(chēng)：
    大鼠不對(duì)稱(chēng)二甲基精氨酸(ADMA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13039r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠不對(duì)稱(chēng)二甲基精氨酸(ADMA)酶聯(lián)免疫試劑盒（CSB-E13039r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ADMA含量。ADMA（非對(duì)稱(chēng)二甲基精氨酸）是一種內(nèi)源性一氧化氮合酶抑制劑，其水平升高與多種疾病如糖尿病視網(wǎng)膜病變和骨關(guān)節(jié)炎相關(guān)。研究顯示，ADMA通過(guò)影響細(xì)胞外基質(zhì)沉積、SOX9降解等途徑，促進(jìn)疾病發(fā)展。靶向DDAH1（ADMA水解酶）可能成為治療相關(guān)疾病的新策略。試劑盒檢測(cè)范圍為1.5 ng/mL-300 ng/mL，本試劑盒適用于心血管疾病模型研究、代謝紊亂機(jī)制探索、腎臟功能評(píng)估等基礎(chǔ)科研實(shí)驗(yàn)，也可用于藥物干預(yù)或基因調(diào)控實(shí)驗(yàn)中ADMA水平變化的動(dòng)態(tài)監(jiān)測(cè)，為動(dòng)物模型病理機(jī)制解析及治療策略開(kāi)發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    ADMA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.5 ng/mL-300 ng/mL
  • 靈敏度：
    0.75 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-93  
    1:2 Average % 92  
    Range % 86-96  
    1:4 Average % 95  
    Range % 90-101  
    1:8 Average % 103  
    Range % 99-106  
  • 回收率：
    The recovery of rat ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 84 80-89  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    300 0.201 0.204 0.203    
    75 0.317 0.345 0.331    
    24 0.523 0.529 0.526 　  
    6 0.952 0.903 0.928 　  
    1.5 1.381 1.316 1.349 　  
    0 2.290 2.212 2.251    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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