Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人Ⅰ型膠原N末端肽(NTX)酶聯(lián)免疫試劑盒（CSB-E09233h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、尿液樣本中的NTX含量。試劑盒檢測(cè)范圍為7.8 nM BCE-500 nM BCE，在骨質(zhì)疏松癥、骨轉(zhuǎn)移癌等骨骼代謝異常相關(guān)研究中具有重要價(jià)值；適用于骨代謝機(jī)制研究、抗骨質(zhì)疏松藥物開發(fā)評(píng)估、骨骼疾病動(dòng)物模型分析等科研領(lǐng)域；支持研究者靈活選擇體液樣本進(jìn)行橫向比對(duì)實(shí)驗(yàn)；為骨代謝相關(guān)基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
縮寫：

NTX
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine
檢測(cè)范圍：

7.8 nM BCE-500 nM BCE
靈敏度：

1.95 nM BCE
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	92-99

回收率：

The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	92	88-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.