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  4. Mouse cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

Mouse cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

  • 中文名稱：
    小鼠Ⅰ型膠原N末端肽(NTX)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09242m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠Ⅰ型膠原N末端肽(NTX)酶聯(lián)免疫試劑盒（CSB-E09242m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NTX含量。小鼠Ⅰ型膠原N末端肽（NTX）是Ⅰ型膠原蛋白的N端肽段，具有特征性的D-Band結(jié)構(gòu)，對骨生物礦化至關(guān)重要。NTX在骨修復(fù)材料中發(fā)揮重要作用，其穩(wěn)定的三螺旋結(jié)構(gòu)和自組裝能力對形成具有生物礦化能力的膠原纖維至關(guān)重要。試劑盒檢測范圍為3.12 nM BCE-200 nM BCE，常用于評估骨質(zhì)疏松、骨折修復(fù)及骨重塑相關(guān)機(jī)制的研究；適用于體外實(shí)驗中對骨代謝疾病模型、藥物干預(yù)效果或骨骼發(fā)育調(diào)控的科研分析，可為骨生物學(xué)、藥理學(xué)及再生醫(yī)學(xué)領(lǐng)域提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
  • 縮寫：
    NTX
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 nM BCE-200 nM BCE
  • 靈敏度：
    0.78 nM BCE
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 88  
    Range % 82-95  
    1:4 Average % 99  
    Range % 94-103  
    1:8 Average % 102  
    Range % 96-108  
  • 回收率：
    The recovery of mouse NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-96  
    EDTA plasma (n=4) 98 95-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nM BCE OD1 OD2 Average Corrected  
    200 2.402 2.435 2.419 2.259  
    100 1.702 1.789 1.746 1.586  
    50 0.966 1.026 0.996 0.836  
    25 0.575 0.596 0.586 0.426  
    12.5 0.349 0.364 0.357 0.197  
    6.25 0.288 0.305 0.297 0.137  
    3.12 0.209 0.201 0.205 0.045  
    0 0.165 0.155 0.160 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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