Human inhibin B,INH-B ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人抑制素B(INH-B)酶聯(lián)免疫試劑盒（CSB-E08149h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INH-B含量。INH-B（抑制素B）是一種由卵巢和睪丸產(chǎn)生的蛋白質(zhì)激素，屬于TGF-β超家族。它在女性中主要由卵巢顆粒細(xì)胞產(chǎn)生，在男性中主要由睪丸支持細(xì)胞合成。INH-B通過負(fù)反饋機(jī)制抑制垂體FSH的分泌，參與調(diào)節(jié)生殖功能和代謝過程。近年來，INH-B在肥胖、糖尿病等代謝性疾病的研究中備受關(guān)注。試劑盒檢測范圍為4 pg/mL-800 pg/mL，生殖系統(tǒng)發(fā)育、性腺功能及生育能力評估等研究；生殖內(nèi)分泌研究、生殖疾病機(jī)制探索（如多囊卵巢綜合征、睪丸功能障礙等）以及動(dòng)物模型評估；科研場景中不同樣本類型的橫向比對分析；生殖健康相關(guān)基礎(chǔ)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

INH-B
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4 pg/mL-800 pg/mL
靈敏度：

2 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	93
	Range %	89-97
1:4	Average %	99
	Range %	96-101
1:8	Average %	92
	Range %	86-96

回收率：

The recovery of human INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	96	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.