Rat inhibin B,INH-B ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠抑制素B(INH-B)酶聯(lián)免疫試劑盒（CSB-E08150r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的INH-B含量。試劑盒檢測(cè)范圍為1 pg/mL-200 pg/mL，本試劑盒通過高特異性抗體與樣本中INH - B的競(jìng)爭(zhēng)性結(jié)合反應(yīng)實(shí)現(xiàn)定量分析，可廣泛應(yīng)用于生殖生物學(xué)研究、激素調(diào)控機(jī)制探索，以及生殖相關(guān)疾病動(dòng)物模型（如睪丸功能障礙、多囊卵巢綜合征等）的科研分析。其檢測(cè)體系經(jīng)過嚴(yán)格優(yōu)化，能夠穩(wěn)定檢測(cè)低濃度樣本，為科研人員提供可靠的數(shù)據(jù)支持，適用于實(shí)驗(yàn)室中對(duì)大鼠模型生殖內(nèi)分泌狀態(tài)的基礎(chǔ)研究與病理機(jī)制評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

INH-B
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1 pg/mL-200 pg/mL
靈敏度：

0.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-106
1:2	Average %	98
	Range %	90-105
1:4	Average %	94
	Range %	89-99
1:8	Average %	96
	Range %	90-100

回收率：

The recovery of rat INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-104
EDTA plasma (n=4)	97	91-106