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  4. Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit

Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit

  • 中文名稱：
    人細(xì)胞間粘附分子3(ICAM-3/CD50)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07949h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人細(xì)胞間粘附分子3(ICAM-3/CD50)酶聯(lián)免疫試劑盒（CSB-E07949h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿,組織勻漿樣本中的ICAM3含量。ICAM3，即細(xì)胞間粘附分子3，是一種跨膜糖蛋白，屬于ICAM家族，主要功能是介導(dǎo)細(xì)胞間的粘附，參與免疫反應(yīng)和細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)。其研究機(jī)制涉及ICAM3與LFA-1等受體的相互作用，以及其在炎癥反應(yīng)和腫瘤浸潤(rùn)中的作用。試劑盒檢測(cè)范圍為31.25 pg/ml- 2000 pg/ml，靈敏度為7.8 pg/ml。為研究免疫相關(guān)疾病機(jī)制、炎癥模型建立、藥物靶點(diǎn)驗(yàn)證等科研需求提供可靠工具，在自身免疫性疾病研究、感染免疫學(xué)實(shí)驗(yàn)及免疫調(diào)節(jié)劑開發(fā)等領(lǐng)域具有廣泛應(yīng)用潛力，滿足實(shí)驗(yàn)室常規(guī)科研場(chǎng)景下的高效檢測(cè)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50
  • 縮寫：
    ICAM3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma,tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/ml - 2000 pg/ml
  • 靈敏度：
    7.8 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    225.927

    236.470

    246.378

    218.347

    231.580

    242.642

    SD

    0.036

    0.044

    0.031

    0.036

    0.046

    0.029

    CV(%)

    4.250

    5.052

    3.865

    4.311

    5.336

    3.532

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-3/CD50 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    89-100

    1:2

    Average %

    88

    Range %

    84-92

    1:4

    Average %

    103

    Range %

    98-107

    1:8

    Average %

    93

    Range %

    89-97

  • 回收率：

    The recovery of human ICAM-3/CD50 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    84

    80-88

    EDTA plasma (n=4)

    96

    91-100

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.752

    2.654

    2.703

    2.548

    1000

    1.981

    2.015

    1.998

    1.843

    500

    1.407

    1.386

    1.397

    1.242

    250

    0.846

    0.872

    0.859

    0.704

    125

    0.529

    0.508

    0.519

    0.364

    62.5

    0.318

    0.321

    0.320

    0.165

    31.25

    0.235

    0.229

    0.232

    0.077

    0

    0.153

    0.157

    0.155

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    ICAM3又稱為白細(xì)胞共同抗原LFA-1，是一種細(xì)胞表面分子，屬于免疫球蛋白超家族，主要在白細(xì)胞、淋巴細(xì)胞、巨噬細(xì)胞等免疫細(xì)胞表面表達(dá)。ICAM3參與調(diào)節(jié)免疫細(xì)胞之間的黏附作用，在細(xì)胞介導(dǎo)免疫應(yīng)答、免疫細(xì)胞遷移和腫瘤免疫等方面發(fā)揮著重要作用。
  • 功能：
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.
  • 基因功能參考文獻(xiàn)：
    1. exploration of the underlying mechanism demonstrated that ICAM3 not only binds to LFA-1 with its extracellular domain and structure protein ERM but also to lamellipodia with its intracellular domain which causes a tension that pulls cells apart (metastasis). PMID: 29729315
    2. Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
    3. ICAM3 acts as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils. PMID: 23775590
    4. this data clearly indicate that ICAM-3 promotes drug resistance via inhibition of apoptosis. PMID: 24177012
    5. Intercellular adhesion molecule (ICAM)-3 mRNA is upregulated in non-adherent endothelial forming cells. PMID: 23144795
    6. Results indicate that the ICAM-3 gene promoter is negatively regulated by RUNX3. PMID: 22479382
    7. analysis of activated apoptotic cells induce dendritic cell maturation via engagement of Toll-like receptor 4 (TLR4), dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN), and beta2 integrins PMID: 22396536
    8. ICAM-3 may be an important adhesion molecule involved in chemotaxis to apoptotic human leukocytes. PMID: 22117198
    9. the molecular basis of allergen-induced Th2 cell polarization PMID: 22205703
    10. the cross-talk between neutrophils and NK cells is mediated by ICAM-3 and CD11d/CD18, respectively. PMID: 21712539
    11. Single nucleotide polymorphisms in ICAM3 gene is associated with lymphoma. PMID: 21239057
    12. induction of morphological polarization in primary T lymphocytes and Jurkat cells enhances Kidins220/ARMS colocalization with ICAM-3 PMID: 21381019
    13. CCR1 antagonist, BX471, did not significantly alter ICAM-3 expression in relapsing-remitting multiple sclerosis patients. PMID: 20086017
    14. ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB PMID: 19956847
    15. no significant risk association was found for SARS infection for the ICAM-3 Asp143Gly SNP. PMID: 19801714
    16. A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targeting PMID: 11784723
    17. identification of DC-SIGN binding sites PMID: 11799126
    18. interactions with DC-SIGN does not promote DC-SIGN mediated HIV-1 transmission PMID: 12021323
    19. Expression of DC-SIGN and its ligand, ICAM-3, is found in substantial amounts only in RA synovium, suggesting that their interaction is implicated in the additional activation of synovial macrophages that leads to the production of EMMPRIN and MMP-1. PMID: 12571844
    20. ICAM-3 is highly expressed on the surface of human eosinophils and has a role in the downregulation of GM-CSF production. PMID: 12743567
    21. Relationship of intercellular adhesion molecule-3 and hepatocyte growth factor with amyloidosis A in chronic renal-failure patients. There was a higher density of intercellular adhesion molecule-3-positive cells in the patients with amyloidosis A. PMID: 14704632
    22. ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via reactive oxygen species-dependent signaling. PMID: 14726630
    23. acts as a costimulating molecule to increase HIV-1 transcription and viral replication, a process allowing productive infection of quiescent CD4+ T lymphocytes. PMID: 15163761
    24. Expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy. PMID: 15880373
    25. The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of dendritic cells. PMID: 17145745
    26. Patients with SARS homozygous for ICAM3 Gly143 showed significant association with higher lactate dehydrogenase levels and lower total white blood cell counts. PMID: 17570115
    27. This is the first case of CD20 positive mycosis fungoides involving a lymph node to be reported in the literature. PMID: 18261116
    28. extended alpha(L)beta(2) with an open headpiece is required for ICAM-3 adhesion PMID: 18354203

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, ICAM family
  • 組織特異性：
    Leukocytes.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5346

    OMIM: 146631

    KEGG: hsa:3385

    STRING: 9606.ENSP00000160262

    UniGene: Hs.654563