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Human malonyl coenzyme A ELISA Kit

  • 中文名稱:
    人丙二酸單酰輔酶A(malonyl CoA)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13877h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人丙二酸單酰輔酶A(malonyl CoA)酶聯(lián)免疫試劑盒(CSB-E13877h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的malonyl CoA含量。Malonyl CoA是一種脂肪酸生物合成的底物,同時(shí)也是脂肪酸氧化過(guò)程中的抑制劑。它在脂肪酸合成中提供2碳單元,并抑制脂肪酸與肉堿結(jié)合,阻止其進(jìn)入線粒體進(jìn)行氧化。研究顯示,Malonyl CoA在調(diào)節(jié)脂肪酸合成與氧化之間起著關(guān)鍵作用,其濃度變化對(duì)酮體生成和能量代謝有重要影響。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL,適用于科研領(lǐng)域中對(duì)肝臟、脂肪組織等代謝活躍器官的功能研究,也可用于探索肥胖、糖尿病等代謝性疾病模型中malonyl CoA的動(dòng)態(tài)變化及分子機(jī)制,為體外實(shí)驗(yàn)樣本的定量分析提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫:
    malonyl CoA
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %86
    Range %80-92
    1:200Average %96
    Range %92-100
    1:400Average %100
    Range %93-110
    1:800Average %91
    Range %85-100
  • 回收率:
    The recovery of human malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9083-95
    EDTA plasma (n=4)9590-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mllOD1OD2AverageCorrected
    402.439 2.252 2.346 2.241
    201.857 1.760 1.809 1.704
    101.340 1.304 1.322 1.217
    50.903 0.890 0.897 0.792
    2.50.515 0.504 0.510 0.405
    1.250.342 0.319 0.331 0.226
    0.6250.213 0.203 0.208 0.103
    00.109 0.101 0.105  
  • 本試劑盒所含材料:
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human Malonyl CoA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • Biotin-labeled Malonyl CoA antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
    • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
    • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

May I know what's the standard in the kit E13877h, recombinant protein?

A:
Thanks for your inquiry.
CSB-E13877h
Standard is small molecule polymer. Pls let me know if you have any further questions. Thank you.