Rat malonyl coenzyme A ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠丙二酸單酰輔酶A(malonyl CoA)酶聯(lián)免疫試劑盒（CSB-E12893r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的malonyl CoA含量。Malonyl CoA即丙二酸單酰輔酶A，是脂肪酸合成過(guò)程中的關(guān)鍵中間產(chǎn)物。其在細(xì)胞能量代謝、脂肪酸合成及氧化平衡等方面發(fā)揮重要作用。研究聚焦于其合成與代謝調(diào)控機(jī)制，如關(guān)鍵酶的作用，以深入了解代謝紊亂疾病發(fā)病機(jī)制并探索潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為1.25 ng/mL-20 ng/mL，能量代謝調(diào)控機(jī)制、肥胖及糖尿病模型研究、心血管疾病相關(guān)脂代謝異常探索、脂肪酸代謝通路研究、藥物干預(yù)效果評(píng)估、營(yíng)養(yǎng)代謝調(diào)控實(shí)驗(yàn)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

malonyl CoA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.25 ng/mL-20 ng/mL
靈敏度：

0.625 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	92-103
1:200	Average %	102
	Range %	100-104
1:400	Average %	93
	Range %	88-97
1:800	Average %	89
	Range %	84-93

回收率：

The recovery of rat malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.