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  4. Human mucosae associated epithelia chemokine,MEC ELISA Kit

Human mucosae associated epithelia chemokine,MEC ELISA Kit

  • 中文名稱：
    人粘膜相關(guān)上皮趨化因子(MEC/CCL28)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07310h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人粘膜相關(guān)上皮趨化因子(MEC/CCL28)酶聯(lián)免疫試劑盒（CSB-E07310h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CCL28含量。CCL28是一種趨化因子，在黏膜組織中表達(dá)，與免疫細(xì)胞的招募和遷移密切相關(guān)。其研究機(jī)制主要聚焦于它和受體的相互作用，這些作用在黏膜免疫、腫瘤微環(huán)境調(diào)節(jié)等過程中發(fā)揮作用，為相關(guān)疾病的治療提供了潛在靶點(diǎn)。試劑盒檢測(cè)范圍為78 pg/ml- 5000 pg/ml，靈敏度為19.5 pg/ml。適用于體外實(shí)驗(yàn)中對(duì)感染性疾病模型、炎癥反應(yīng)研究或免疫調(diào)控機(jī)制探索中的MEC/CCL28表達(dá)水平分析，例如評(píng)估實(shí)驗(yàn)條件下趨化因子分泌動(dòng)態(tài)或驗(yàn)證基因干預(yù)效果。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C C motif chemokine ligand 28 ELISA Kit; C-C motif chemokine 28 ELISA Kit; CC chemokine CCL28 ELISA Kit; CCK 1 ELISA Kit; CCK1 ELISA Kit; CCK1 protein ELISA Kit; CCL 28 ELISA Kit; CCL28 ELISA Kit; CCL28_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 28 ELISA Kit; chemokine (C-C motif) ligand 28 splice variant chi ELISA Kit; MEC ELISA Kit; Mucosae associated epithelial chemokine ELISA Kit; Mucosae-associated epithelial chemokine ELISA Kit; Protein CCK1 ELISA Kit; SCYA28 ELISA Kit; Small inducible cytokine A28 [Precursor] ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 28 ELISA Kit; Small-inducible cytokine A28 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    78 pg/ml - 5000 pg/ml
  • 靈敏度：
    19.5 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MEC/CCL28 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    85-98

    1:2

    Average %

    97

    Range %

    90-105

    1:4

    Average %

    98

    Range %

    92-101

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of human MEC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    85-105

    EDTA plasma (n=4)

    99

    94-103

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.534

    2.634

    2.584

    2.451

    2500

    1.953

    1.867

    1.910

    1.777

    1250

    1.284

    1.308

    1.296

    1.163

    625

    0.737

    0.699

    0.718

    0.585

    312

    0.457

    0.431

    0.444

    0.311

    156

    0.271

    0.253

    0.262

    0.129

    78

    0.184

    0.184

    0.184

    0.051

    0

    0.137

    0.129

    0.133

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
  • 基因功能參考文獻(xiàn)：
    1. this review discusses the role of CCL28 in innate and adaptive immunity PMID: 28843907
    2. Results show that CCL28 expression was up-regulated under hypoxic condition in lung adenocarcinoma cells. Besides other effects on tumor biology such as immunosuppression, CCL28 could promote angiogenesis in lung adenocarcinoma by directly activating its receptor, CCR3, on microvascular endothelial cells. PMID: 27250766
    3. our results show for the first time that CCL28 contributes to breast cancer progression through the ERK/MAPKmediated anti-apoptotic and metastatic signaling pathway. Antagonists of CCL28 and the MAPK signaling pathway may be used synergistically to treat breast cancer patients. PMID: 28713975
    4. HCC recruits Tregs to promote angiogenesis under hypoxic condition by upregulating CCL28 expression. These findings establish a link between Tregs and hypoxia in HCC growth and may provide a new potential therapeutic target for treating HCC. PMID: 27716621
    5. CCL28 was absent in saliva of primary Sjogren's syndrome patients. This finding did not correlate with salivary IgA levels. PMID: 25567740
    6. disease severity of atopic dermatitis in children is not correlated to the level of CCL28, but rather related to that of total IgE PMID: 26642722
    7. CCL28-CCR3 interactions are involved in the homeostatic trafficking of CD4(+) T cells to the upper airways. PMID: 24917456
    8. High CCL28 gene methylation is associated with gastric tumor aggressiveness. PMID: 25740824
    9. CCL28 is a potent growth-promoting factor with the ability to support the in vitro and in vivo functional properties of cultured human hematopoietic cells. PMID: 23509159
    10. These results indicate a role for IL-17A in the human lung by enhancing the expression of CCL28 and hence driving the recruitment of IgE-secreting B cells. PMID: 21447959
    11. tumour hypoxia promotes the recruitment of regulatory T (T(reg)) cells through induction of expression of the chemokine CC-chemokine ligand 28 (CCL28), which, in turn, promotes tumour tolerance and angiogenesis PMID: 21753853
    12. CCL28 may play dual roles in mucosal immunity as a chemoattractant for cells expressing CCR10 and/or CCR3 such as plasma cells and also as a broad-spectrum antimicrobial protein secreted into low-salt body fluids. PMID: 12538707
    13. CCR10 and its mucosal epithelial ligand CCL28 have roles in the migration of circulating IgA plasmablasts PMID: 12671049
    14. CCL28 produced by keratinocytes is mediated by different signal pathways from CCL27, and both CCL27 and CCL28 are involved in the pathogenesis of inflammatory skin diseases. PMID: 16433680
    15. CCL28 is one link between microbial insult and the exacerbation of pathologies such as asthma, through an NFkappaB-dependent mechanism PMID: 16581045
    16. Expression of CCL28 by epithelial cells from chronically inflamed liver in response to microbial products or interleukin-1 provides a signal to localize CCR10-expressing regulatory T cells at mucosal surfaces. PMID: 16785557
    17. CCL28 mediates mucosal immunity in HIV exposure and infection PMID: 17912348
    18. These studies are the first to show increased CCL28 production during gastrointestinal infection in humans and provide an explanation for the large influx of IgA-secreting cells to the gastric mucosa in H. pylori-infected individuals. PMID: 18426876
    19. There was a robust migration of specific IgA- and IgM-antibody-secreting cells induced by Salmonella vaccination toward the mucosal chemokines CCL25 and CCL28 PMID: 19003934
    20. data reinforce concept that CCL28 may contribute to the pathogenesis of atopic dermatitis probably through selective migration & infiltration of effector/memory T-helper-2 cells in the skin; CCL28 may represent a prognostic marker for disease severity PMID: 19659860

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leu
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17700

    OMIM: 605240

    KEGG: hsa:56477

    STRING: 9606.ENSP00000354416

    UniGene: Hs.656904