Human oxidized high density lipoprotein (Ox-HDL)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人氧化高密度脂蛋白(Ox-HDL)酶聯(lián)免疫試劑盒（CSB-E16552h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的Ox-HDL含量。Ox - HDL指氧化型高密度脂蛋白，HDL本具抗動脈粥樣硬化等有益作用，但氧化后功能改變。研究機(jī)制上，Ox - HDL可通過多種途徑影響細(xì)胞功能，比如促使巨噬細(xì)胞攝取脂質(zhì)形成泡沫細(xì)胞，參與動脈粥樣硬化等疾病的發(fā)生發(fā)展。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，可靈活應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域，如探究氧化應(yīng)激狀態(tài)下脂蛋白代謝機(jī)制、評估抗氧化藥物干預(yù)效果、建立疾病模型生物標(biāo)志物分析體系等；滿足細(xì)胞實(shí)驗(yàn)、動物模型及臨床前研究中對Ox-HDL的精準(zhǔn)定量需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Ox-HDL
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
Range %	80-98
1:200	Average %	96
Range %	91-100
1:400	Average %	94
Range %	90-105
1:800	Average %	92
Range %	86-98

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.