Mouse oxidized high density lipoprotein (Ox-HDL)ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠氧化高密度脂蛋白(Ox-HDL)酶聯(lián)免疫試劑盒（CSB-EQ027598MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Ox-HDL含量。Ox - HDL是氧化型高密度脂蛋白。在生理背景下，HDL氧化后功能改變。研究機制方面，其可影響膽固醇逆向轉(zhuǎn)運等過程，還能與細胞表面受體相互作用，引發(fā)一系列細胞內(nèi)信號轉(zhuǎn)導變化，與動脈粥樣硬化等疾病的發(fā)生發(fā)展密切相關(guān)。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于心血管疾病動物模型研究、抗氧化藥物療效評估、氧化應激相關(guān)機制探索等科研領(lǐng)域。通過檢測不同樣本類型中的 Ox-HDL 含量，可為脂蛋白氧化損傷機制研究及潛在干預策略開發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Ox-HDL
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-99
1:200	Average %	94
	Range %	90-98
1:400	Average %	98
	Range %	94-104
1:800	Average %	84
	Range %	80-88

回收率：

The recovery of mouse Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-97
EDTA plasma (n=4)	99	95-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.