Human oxyntomodulin ELISA Kit
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中文名稱:人胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E12948h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒(CSB-E12948h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的OXM含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL,適用于體外研究場(chǎng)景下OXM的表達(dá)水平分析,可為能量代謝調(diào)控機(jī)制研究、胃腸道功能探索及代謝性疾病分子機(jī)制研究提供可靠數(shù)據(jù)支持,滿足基礎(chǔ)科研與藥物研發(fā)中對(duì)OXM定量檢測(cè)的需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):OXM
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, cell culture supernates, urine, tissue homogenates
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檢測(cè)范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Metabolism
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 83-94 1:2 Average % 84 Range % 80-89 1:4 Average % 95 Range % 89-99 1:8 Average % 92 Range % 88-96 -
回收率:
The recovery of human Oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-99 EDTA plasma (n=4) 98 92-103 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.263 2.181 2.222 2.042 10 1.761 1.791 1.776 1.596 5 1.263 1.201 1.232 1.052 2.5 0.766 0.723 0.745 0.565 1.25 0.443 0.417 0.430 0.250 0.625 0.274 0.253 0.264 0.084 0.312 0.267 0.282 0.275 0.095 0 0.181 0.179 0.180 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- NDRG1 Activity in Fat Depots Is Associated With Type 2 Diabetes and Impaired Incretin Profile in Patients With Morbid Obesity Iurii Stafeev,Frontiers in endocrinology,2022
- РЕМИССИЯ САХАРНОГО ДИАБЕТА 2 ТИПА ПРИ ИСПОЛЬЗОВАНИИ МЕДИКАМЕНТОЗНОГО (ЛИРАГЛУТИД) И БАРИАТРИЧЕСКИХ МЕТОДОВ ЛЕЧЕНИЯ: СРОКИ НАСТУПЛЕНИЯ, ПРОГНОСТИЧЕСКИЕ ФАКТОРЫ, МЕХАНИЗМЫ ИА СКЛЯНИК,/,2020
- Low AS160 and high SGK basal phosphorylation associates with impaired incretin profile and type 2 diabetes in adipose tissue of obese patients Iurii S. Stafeev, et al,Diabetes Research and Clinical Practice,2019
- Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human Bak MJ et al,Eur J Endocrinol,2014
- Effects of a brown beans evening meal on metabolic risk markers and appetite regulating hormones at a subsequent standardized breakfast: a randomized cross-over study Nilsson A et al,PLoS One,2013
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