Rat oxyntomodulin ELISA Kit
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中文名稱(chēng):大鼠胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E12949r
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒(CSB-E12949r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的OXM含量。OXM(胰高血糖素樣肽 -1/胰高血糖素雙受體激動(dòng)劑)具有獨(dú)特背景。它涉及調(diào)節(jié)能量代謝、血糖的生理機(jī)制。研究表明,OXM可作用于相應(yīng)受體,促進(jìn)胰島素分泌、抑制胰高血糖素分泌、延緩胃排空,有望用于肥胖、糖尿病等疾病的治療。試劑盒檢測(cè)范圍為125 pg/mL-8000 pg/mL,適用于大鼠模型中OXM表達(dá)水平與胃腸功能、代謝調(diào)控的相關(guān)性研究,可應(yīng)用于藥物干預(yù)、飲食調(diào)控或病理狀態(tài)下OXM動(dòng)態(tài)變化的科研分析。樣本處理簡(jiǎn)便,標(biāo)準(zhǔn)曲線覆蓋生理及病理濃度范圍,滿足基礎(chǔ)研究中血清、血漿等液態(tài)樣本及胃腸組織勻漿的檢測(cè)需求,為消化系統(tǒng)與代謝調(diào)控領(lǐng)域的分子機(jī)制探索提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):OXM
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種屬:Rattus norvegicus (Rat)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:125 pg/mL-8000 pg/mL
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靈敏度:31.25 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Metabolism
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 81-92 1:2 Average % 96 Range % 90-102 1:4 Average % 97 Range % 92-104 1:8 Average % 93 Range % 90-96 -
回收率:
The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 87-100 EDTA plasma (n=4) 99 95-104 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 8000 2.356 2.378 2.367 2.252 4000 2.121 2.161 2.141 2.026 2000 1.757 1.798 1.778 1.663 1000 1.343 1.391 1.367 1.252 500 0.851 0.899 0.875 0.760 250 0.502 0.539 0.521 0.406 125 0.272 0.291 0.282 0.167 0 0.114 0.116 0.115 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Angptl8 mediates food-driven resetting of hepatic circadian clock in mice Chen S, et al,Nature Communications,2019
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