Human procollagen type III,HPCⅢ ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人Ⅲ型前膠原（HPCⅢ）酶聯(lián)免疫試劑盒（CSB-E11989h）為雙抗夾心法ELISA試劑盒，定量檢測血清樣本中的HPCⅢ含量。試劑盒檢測范圍為25 ng/mL-300 ng/mL，適用于體外研究領(lǐng)域，如通過檢測動物模型或細(xì)胞培養(yǎng)上清中的HPCⅢ水平，探索肝纖維化發(fā)生機(jī)制、篩選抗纖維化藥物活性，或用于基礎(chǔ)醫(yī)學(xué)研究中組織修復(fù)與纖維化相關(guān)疾病的病理分析。為肝病研究、藥物開發(fā)及分子生物學(xué)實驗提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
縮寫：

HPCⅢ
種屬：

Homo sapiens (Human)
樣本類型：

serum
檢測范圍：

25 ng/mL-300 ng/mL
靈敏度：

25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	93
	Range %	90-96
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	97	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.