Rat procollagen type III (HPCⅢ) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠Ⅲ型前膠原（HPCⅢ）酶聯(lián)免疫試劑盒（CSB-E15810r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的HPCⅢ含量。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，在肝纖維化、肺纖維化等病理過(guò)程中可作為重要生物標(biāo)志物，為相關(guān)疾病機(jī)制研究提供實(shí)驗(yàn)依據(jù)；適用于體外實(shí)驗(yàn)環(huán)境下對(duì)纖維化疾病模型、藥物干預(yù)效果或毒性作用研究中HPCⅢ的動(dòng)態(tài)監(jiān)測(cè)，為科研人員探究組織修復(fù)機(jī)制及抗纖維化治療策略提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
縮寫：

HPCⅢ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	84
	Range %	80-88
1:10	Average %	97
	Range %	91-102
1:20	Average %	97
	Range %	92-104
1:40	Average %	93
	Range %	90-96

回收率：

The recovery of rat HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	97	92-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.