Human pyridinoline (PYD) ELISA Kit

產(chǎn)品詳情

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文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人膠原吡啶交聯(lián)(PYD)酶聯(lián)免疫試劑盒（CSB-E09702h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的PYD含量。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于骨代謝機(jī)制研究、抗骨質(zhì)疏松藥物篩選、關(guān)節(jié)炎動物模型評估等科研領(lǐng)域。特別針對尿液樣本的兼容性設(shè)計，為無創(chuàng)性骨代謝動態(tài)監(jiān)測提供了便捷方案，可滿足體外培養(yǎng)體系檢測、疾病模型縱向觀察等實驗需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

PYD
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-100
1:2	Average %	92
	Range %	86-99
1:4	Average %	87
	Range %	80-94
1:8	Average %	97
	Range %	92-102

回收率：

The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-99
EDTA plasma (n=4)	98	92-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.