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Mouse pyridinoline (PYD) ELISA Kit

  • 中文名稱:
    小鼠膠原吡啶交聯(lián)(PYD)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E15010m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠膠原吡啶交聯(lián)(PYD)酶聯(lián)免疫試劑盒(CSB-E15010m)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的PYD含量。小鼠膠原吡啶交聯(lián)(PYD)是一種通過吡啶交聯(lián)劑對(duì)膠原蛋白進(jìn)行交聯(lián)的技術(shù),旨在改善膠原蛋白的物理和生物性能。該技術(shù)通過交聯(lián)反應(yīng)增加膠原蛋白的穩(wěn)定性和機(jī)械強(qiáng)度,廣泛應(yīng)用于組織工程和再生醫(yī)學(xué)領(lǐng)域,以促進(jìn)組織修復(fù)和再生。試劑盒檢測(cè)范圍為50 ng/mL-800 ng/mL,適用于骨代謝機(jī)制研究、骨質(zhì)疏松動(dòng)物模型評(píng)估、抗骨吸收藥物開發(fā)等科研領(lǐng)域,為骨代謝相關(guān)基礎(chǔ)研究提供高效可靠的定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    PYD
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    50 ng/mL-800 ng/mL
  • 靈敏度:
    50 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-98  
    1:2 Average % 100  
    Range % 92-106  
    1:4 Average % 85  
    Range % 80-90  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率:
    The recovery of mouse PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 88-97  
    EDTA plasma (n=4) 93 91-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    800 0.147 0.145 0.146    
    400 0.241 0.254 0.248    
    200 0.534 0.554 0.544    
    100 1.102 1.139 1.121    
    50 2.318 2.278 2.298    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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