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  4. Human soluble amyloid precursor protein α (sAPPα) ELISA kit

Human soluble amyloid precursor protein α (sAPPα) ELISA kit

  • 中文名稱：
    人可溶性淀粉酶前體蛋白α(sAPPα)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ027464HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人可溶性淀粉酶前體蛋白α(sAPPα)酶聯(lián)免疫試劑盒（CSB-EQ027464HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液、腦脊液（CSF）樣本中的sAPPα含量。sAPPα是一個(gè)備受關(guān)注的靶點(diǎn)。其它在生理過(guò)程中有重要作用。研究機(jī)制上，目前認(rèn)為其與神經(jīng)系統(tǒng)等功能相關(guān)，參與神經(jīng)保護(hù)、突觸可塑性調(diào)節(jié)等過(guò)程，深入探索其作用機(jī)制有望為相關(guān)神經(jīng)疾病治療帶來(lái)新突破。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL，適用于神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究，包括疾病模型中的蛋白代謝分析、細(xì)胞實(shí)驗(yàn)中的分泌動(dòng)態(tài)監(jiān)測(cè)，以及藥物干預(yù)下sAPPα水平變化的評(píng)估等科研場(chǎng)景，為探索神經(jīng)系統(tǒng)疾病機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    soluble amyloid precursor protein alpha, sAPP alpha
  • 縮寫：
    sAPPα
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF)
  • 檢測(cè)范圍：
    12.5 ng/mL-800 ng/mL
  • 靈敏度：
    3.12 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human sAPPα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %82-94
    1:2Average %101
    Range %90-105
    1:4Average %91
    Range %85-94
    1:8Average %93
    Range %85-97
  • 回收率：
    The recovery of human sAPPα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9485-97
    EDTA plasma (n=4)9790-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8001.822 1.902 1.862 1.729
    4001.454 1.351 1.403 1.270
    2000.859 0.901 0.880 0.747
    1000.537 0.548 0.543 0.410
    500.339 0.345 0.342 0.209
    250.234 0.222 0.228 0.095
    12.50.166 0.172 0.169 0.036
    00.132 0.134 0.133
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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