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Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

  • 中文名稱:
    小鼠金屬肽酶含血小板反應(yīng)蛋白5(ADAMTS5)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL001312MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠金屬肽酶含血小板反應(yīng)蛋白5(ADAMTS5)酶聯(lián)免疫試劑盒(CSB-EL001312MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ADAMTS5含量。ADAMTS5 是重要的藥物靶點(diǎn)。它屬去整合素樣金屬蛋白酶家族,與軟骨破壞密切相關(guān),在骨關(guān)節(jié)炎等疾病發(fā)病機(jī)制中發(fā)揮關(guān)鍵作用。研究聚焦其在疾病進(jìn)程中的作用機(jī)制,期望通過抑制它的活性來開發(fā)治療骨關(guān)節(jié)炎等病癥的藥物。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,適用于科研領(lǐng)域中對小鼠ADAMTS5表達(dá)調(diào)控的機(jī)制研究,如關(guān)節(jié)炎模型中的基質(zhì)代謝分析、炎癥相關(guān)信號通路探索或藥物干預(yù)效果評估。支持科研人員在體外實(shí)驗(yàn)中系統(tǒng)量化ADAMTS5在生理或病理狀態(tài)下的動(dòng)態(tài)變化,為疾病機(jī)理研究及生物標(biāo)志物篩選提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Adamts5 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 5 ELISA Kit; ADAM-TS 5 ELISA Kit; ADAM-TS5 ELISA Kit; ADAMTS-5 ELISA Kit; EC 3.4.24.- ELISA Kit; ADMP-2 ELISA Kit; Aggrecanase-2 ELISA Kit; Implantin ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %80-98
    1:2Average %92
    Range %81-105
    1:4Average %94
    Range %92-107
    1:8Average %95
    Range %86-106
  • 回收率:
    The recovery of mouse ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9184-98
    EDTA plasma (n=4)9692-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.423 2.414 2.419 2.236
    201.621 1.557 1.589 1.406
    100.904 0.925 0.915 0.732
    50.543 0.558 0.551 0.368
    2.50.382 0.372 0.377 0.194
    1.250.299 0.305 0.302 0.119
    0.6250.247 0.256 0.252 0.069
    00.181 0.185 0.183
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that shows proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. Participates in the development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
  • 基因功能參考文獻(xiàn):
    1. ADAMTS5 plays a functional role in development of brown adipose tissue and browning of white adipose tissue. PMID: 28702327
    2. Energy expenditure and thermogenesis were not significantly different between KO and WT ADAMTS5-J mice (in contrast to somewhat enhanced levels in ADAMTS5-P mice). Insulin sensitivity was improved in the ADAMTS5-J KO mice, and they were protected against non-alcoholic steatohepatitis in the DIO model PMID: 29293679
    3. Adamts5(-/-) mice were protected from hepatic mitochondrial dysfunction, as indicated by increased mitochondrial respiratory chain complex activity, higher ATP levels and higher expression of antioxidant enzymes. Absence of ADAMTS5 preserves liver integrity in a diet-induced obesity model. PMID: 27254774
    4. research emphasises the importance of ADAMTS5 expression in the control of influenza virus infection and highlights the potential for development of ADAMTS5-based therapeutic strategies to reduce morbidity and mortality PMID: 27855162
    5. TS5 protein functions to suppress glucose uptake in adipose-derived stromal cells and thereby inhibits the synthesis, and promotes the intracellular degradation of Acan and Vcan by an ADAMTS other than TS5. PMID: 25840345
    6. Data suggest that ADAMTS-5 oligomerization is required for full aggrecanase activity in vitro and in situ (as seen in knee joint of mouse model of inflammatory arthritis); thus, blocking oligomerization inhibits ADAMTS-5 activity. PMID: 26668318
    7. aggrecan and brevican proteolysis is compensated in Adamts4-/- or Adamts5-/- mice by ADAMTS proteoglycanase family members but a threshold of versican proteolysis is sensitive to the loss of a single ADAMTS proteoglycanase during spinal cord injury PMID: 25101296
    8. The present study reveals ADAMT-5 expression by mast cells(MCs) and that MC activation regulates the expression of the protease, thus implicating the ADAMT-5 of protease in MC function. PMID: 23154421
    9. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI. PMID: 23562508
    10. RelA/p65 is a potent transcriptional activator of ADAMTS5 in chondrocytes during osteoarthritis development. PMID: 23963448
    11. Repair of biomechanically compromised tendons exhibiting midsubstance chondroid accumulation requires ADAMTS5. PMID: 23754494
    12. this study identified, for the first time, several genes that have an ADAMTS-5-independent role in osteoarthritis(OA), identifying them as possible OA initiation candidates. PMID: 23436205
    13. The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
    14. Versican processing by ADAMTS5 and ADAMTS15 contribute to muscle fiber formation. PMID: 23233679
    15. the first evidence implicating ADAMTS-5 in the regulation of proteoglycan turnover and lipoprotein retention in atherosclerosis. PMID: 22493487
    16. The role of ADAMTS5 in tendon is to remove pericellular and interfibrillar aggrecan to maintain the molecular architecture responsible for normal tissue function. PMID: 21928430
    17. Adamts5 induction in joint components other than cartilage, and its post-translational activation by PACE4 and/or furin may be important in the pathophysiology of arthritis. PMID: 21800360
    18. Matrilin-4 is processed by ADAMTS-5 in late Golgi vesicles present in growth plate chondrocytes of defined differentiation state PMID: 21539915
    19. a physiological function of ADAMTS5 in dermal fibroblasts is to maintain optimal versican content and PCM volume by continually trimming versican in hyaluronan-versican aggregates. PMID: 21828051
    20. Altered versican cleavage in ADAMTS5 deficient mice; a novel etiology of myxomatous valve disease PMID: 21749862
    21. ADAMTS5 ablation did not eliminate aggrecanase activity from the articular cartilage but blocked fibrosis and resulted in the accumulation of aggrecan in the articular cartilage. PMID: 21337391
    22. extracellular matrix degrading enzyme PMID: 11831030
    23. ADAMTS1, ADAMTS4, and ADAMTS5 are expressed in patterns that relate to the expression pattern of versican in granulosa cells of small follicles, expanded cumulus cell-oocyte complexes, and endothelial cells of the ovary. PMID: 15659705
    24. After surgically induced joint instability, there was significant reduction in the severity of cartilage destruction in the ADAMTS5 knockout mice compared with wild-type mice PMID: 15800624
    25. ADAMTS5 is the major aggrecanase in mouse cartilage, both in vitro and in a mouse model of inflammatory arthritis PMID: 15800625
    26. ADAMTS-5 is entirely responsible for cleavage in the interglobular domain, but cleavage in the chondroitin sulfate-rich region may be driven by ADAMTS-4 PMID: 17255106
    27. aggrecan loss with aggrecan processing in mice with single and double deletions of ADAMTS-4 and -5 activity (Deltacat) PMID: 17938173
    28. Deletion of ADAMTS-4/5 provided significant protection against proteoglycan degradation ex vivo and decreased the severity of murine osteoarthritis PMID: 17968948
    29. Data show that expression of Adamts5 during neuromuscular development and in smooth muscle cells coincides with the broadly distributed proteoglycan versican, an ADAMTS5 substrate. PMID: 19250981
    30. Fibroblast growth factor 2 is an intrinsic chondroprotective agent that suppresses ADAMTS-5 and delays cartilage degradation in murine osteoarthritis. PMID: 19565481
    31. The occurrence of less severe osteoarthritis-like cartilage destruction in both syndecan-4-deficient mice and syndecan-4-specific antibody-treated wild-type mice results from a marked decrease in ADAMTS-5 activity. PMID: 19684582
    32. show that combinatorial mouse alleles for the secreted metalloproteases Adamts5, Adamts20 (bt), and Adamts9 result in fully penetrant soft-tissue syndactyly. PMID: 19922873

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  • 亞細(xì)胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性:
    Expressed in skeletal muscle.
  • 數(shù)據(jù)庫鏈接: